Literature DB >> 6275834

Cyclic nucleotide phosphodiesterase of rat pancreatic islets. Effects of Ca2+, calmodulin and trifluoperazine.

M C Sugden, S J Ashcroft.   

Abstract

Cyclic nucleotide phosphodiesterase activity towards cyclic AMP and cyclic GMP was studied in extracts of rat islets of Langerhans. Biphasic Eadie plots [Eadie (1942) J. Biol. Chem. 146, 85-93] were obtained with either substrate suggesting the presence of both 'high'- and 'low'-Km components. The apparent Km values were 6.2 +/- 0.5 (n = 8) microM and 103.4 +/- 13.5 (6) microM for cyclic AMP and 3.6 +/- 0.3 (12) microM and 61.4 +/- 7.5 (13) microM for cyclic GMP. With cyclic AMP as substrate, phosphodeisterase activity was increased by calmodulin and Ca2+ and decreased by trifluoperazine, a specific inhibitor of calmodulin. With cyclic GMP as substrate, phosphodiesterase activity was decreased by omission of Ca2+ or addition of trifluoperazine. Addition of exogenous calmodulin had no effect on activity. The data suggest that Ca2+ may influence the islet content of cyclic AMP and cyclic GMP via effects on calmodulin-dependent cyclic nucleotide phosphodiesterase(s).

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Year:  1981        PMID： 6275834      PMCID： PMC1163146          DOI： 10.1042/bj1970459

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  Cyclic nucleotide phosphodiesterase activity in normal mouse pancreatic islets.

Authors:  S J.H. Ashcroft; P J. Randle; I -B. Täljedal
Journal:  FEBS Lett       Date:  1972-02-15       Impact factor: 4.124

2.  Identification of the Ca2+-dependent modulator protein as the fourth subunit of rabbit skeletal muscle phosphorylase kinase.

Authors:  P Cohen; A Burchell; J G Foulkes; P T Cohen; T C Vanaman; C Nairn
Journal:  FEBS Lett       Date:  1978-08-15       Impact factor: 4.124

3.  Factors governing glucose induced elevation of cyclic 3'5' AMP levels in pancreatic islets.

Authors:  W S Zawalich; R C Karl; J A Ferrendelli; F M Matschinsky
Journal:  Diabetologia       Date:  1975-06       Impact factor: 10.122

4.  The adenylate cyclase-cyclic AMP system in islets of Langerhans and its role in the control of insulin release.

Authors:  G W Sharp
Journal:  Diabetologia       Date:  1979-05       Impact factor: 10.122

5.  Insulin release, cGMP, cAMP, and membrane potential in acetylcholine-stimulated islets.

Authors:  E Gagerman; L A Idahl; H P Meissner; I B Täljedal
Journal:  Am J Physiol       Date:  1978-11

6.  Insulin release by isolated pancreatic islets of the mouse incubated in vitro.

Authors:  E Coll-Garcia; J R Gill
Journal:  Diabetologia       Date:  1969-04       Impact factor: 10.122

7.  Cyclic 3',5'-nucleotide phosphodiesterase. Demonstration of an activator.

Authors:  W Y Cheung
Journal:  Biochem Biophys Res Commun       Date:  1970-02-06       Impact factor: 3.575

8.  Cyclic nucleotides in pancreatic islets. Tolbutamide- and arginine-induced insulin release.

Authors:  M A Charles; J Lawecki; A L Steiner; G M Grodsky
Journal:  Diabetes       Date:  1976-04       Impact factor: 9.461

9.  The effect of starvation on phosphodiesterase activity and the content of adenosine 3' :5'-cyclic monophosphate in isolated mouse pancreatic islets.

Authors:  K Capito; C J Hedeskov
Journal:  Biochem J       Date:  1974-09       Impact factor: 3.857

Review 10.  Glucoreceptor mechanisms and the control of insulin release and biosynthesis.

Authors:  S J Ashcroft
Journal:  Diabetologia       Date:  1980-01       Impact factor: 10.122
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  13 in total

Review 1.  Calmodulin and pancreatic B-cell function.

Authors:  I Valverde; W J Malaisse
Journal:  Experientia       Date:  1984-10-15

Review 2.  The role of cyclic AMP in insulin release.

Authors:  W J Malaisse; F Malaisse-Lagae
Journal:  Experientia       Date:  1984-10-15

3.  Increase in insulin release from rat pancreatic islets by quinolone antibiotics.

Authors:  N Maeda; T Tamagawa; I Niki; H Miura; K Ozawa; G Watanabe; K Nonogaki; K Uemura; A Iguchi
Journal:  Br J Pharmacol       Date:  1996-01       Impact factor: 8.739

4.  Effect of type-selective inhibitors on cyclic nucleotide phosphodiesterase activity and insulin secretion in the clonal insulin secreting cell line BRIN-BD11.

Authors:  M Ahmad; Y H Abdel-Wahab; R Tate; P R Flatt; N J Pyne; B L Furman
Journal:  Br J Pharmacol       Date:  2000-03       Impact factor: 8.739

Review 5.  Mechanisms of action of glucagon-like peptide 1 in the pancreas.

Authors:  Máire E Doyle; Josephine M Egan
Journal:  Pharmacol Ther       Date:  2006-12-28       Impact factor: 12.310

6.  Calmodulin in various insulin secreting cell types.

Authors:  D Janjic; C B Wollheim; A E Renold
Journal:  J Endocrinol Invest       Date:  1985-10       Impact factor: 4.256

7.  Protein phosphorylation in the regulation of insulin secretion: the use of site-directed inhibitory peptides in electrically permeabilised islets of Langerhans.

Authors:  H Basudev; P M Jones; S L Howell
Journal:  Acta Diabetol       Date:  1995-03       Impact factor: 4.280

8.  Correlation of Ca2+-and calmodulin-dependent protein kinase activity with secretion of insulin from islets of Langerhans.

Authors:  J R Colca; C L Brooks; M Landt; M L McDaniel
Journal:  Biochem J       Date:  1983-06-15       Impact factor: 3.857

9.  Effects of type-selective phosphodiesterase inhibitors on glucose-induced insulin secretion and islet phosphodiesterase activity.

Authors:  R Shafiee-Nick; N J Pyne; B L Furman
Journal:  Br J Pharmacol       Date:  1995-08       Impact factor: 8.739

10.  Cyclic AMP-dependent protein phosphorylation and insulin secretion in intact islets of Langerhans.

Authors:  M R Christie; S J Ashcroft
Journal:  Biochem J       Date:  1984-02-15       Impact factor: 3.857
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