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Literature DB >> 6275119

Genetic recombination of bacteriophage T7 in vivo studied by use of a simple physical assay.

Abstract

A new physical method was developed to assay genetic recombination of phage T7 in vivo. The assay utilized T7 mutants that carry unique restriction sites and was based on the detection of a new restriction fragment generated by recombination. Using this assay, we reexamined the genetic requirements for recombination of T7 DNA. Our results were in total agreement with previous findings in that recombination required the products of genes 3 (endonuclease), 4 (primase), 5 (DNA polymerase), and 6 (exonuclease). Recombination was found to be independent of DNA ligase and DNA packaging and maturation functions.

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Year: 1981 PMID： 6275119 PMCID： PMC256695

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

30 in total

1. MOLECULAR MECHANISMS OF GENETIC RECOMBINATION IN BACTERIOPHAGE. II. JOINING OF PARENTAL DNA MOLECULES OF PHAGE T4.

Authors: J I TOMIZAWA; N ANRAKU
Journal: J Mol Biol Date: 1964-04 Impact factor: 5.469

2. Restriction assay for integrative recombination of bacteriophage lambda DNA in vitro: requirement for closed circular DNA substrate.

Authors: K Mizuuchi; H A Nash
Journal: Proc Natl Acad Sci U S A Date: 1976-10 Impact factor: 11.205

3. The purification of restriction endonuclease EcoRI by precipitation involving polyethyleneimine.

Authors: A H Bingham; A F Sharman; T Atkinson
Journal: FEBS Lett Date: 1977-04-15 Impact factor: 4.124

4. Variations in sedimentation patterns among deoxyribonucleic acids synthesized after infection of Escherichia coli by different amber mutants of bacteriophage T7.

Authors: R Hausmann; K LaRue
Journal: J Virol Date: 1969-02 Impact factor: 5.103

5. Variations in genetic recombination due to amber mutations in T4D bacteriophage.

Authors: H Berger; A J Warren; K E Fry
Journal: J Virol Date: 1969-02 Impact factor: 5.103

6. Repair and recombination in phage T4. I. Genes affecting recombination.

Authors: H Bernstein
Journal: Cold Spring Harb Symp Quant Biol Date: 1968

7. Bacteriophage T7 morphogenesis: phage-related particles in cells infected with wild-type and mutant T7 phage.

Authors: G S Roeder; P D Sadowski
Journal: Virology Date: 1977-01 Impact factor: 3.616

8. EcoRI-sensitive mutation of T7 phage.

Authors: Y Tsujimoto; H Ogawa
Journal: Mol Gen Genet Date: 1977-01-18

9. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Authors: M W McDonell; M N Simon; F W Studier
Journal: J Mol Biol Date: 1977-02-15 Impact factor: 5.469

7. Functional analysis of the ABF1-binding sites within the Ya regions of the MATa and HMRa loci of Saccharomyces cerevisiae.

Authors: L D McBroom; P D Sadowski
Journal: Curr Genet Date: 1995-06 Impact factor: 3.886

8. Endonuclease VII resolves Y-junctions in branched DNA in vitro.

Authors: F Jensch; B Kemper
Journal: EMBO J Date: 1986-01 Impact factor: 11.598

8 in total

Genetic recombination of bacteriophage T7 in vivo studied by use of a simple physical assay.

1. MOLECULAR MECHANISMS OF GENETIC RECOMBINATION IN BACTERIOPHAGE. II. JOINING OF PARENTAL DNA MOLECULES OF PHAGE T4.

2. Restriction assay for integrative recombination of bacteriophage lambda DNA in vitro: requirement for closed circular DNA substrate.

3. The purification of restriction endonuclease EcoRI by precipitation involving polyethyleneimine.

4. Variations in sedimentation patterns among deoxyribonucleic acids synthesized after infection of Escherichia coli by different amber mutants of bacteriophage T7.

5. Variations in genetic recombination due to amber mutations in T4D bacteriophage.

6. Repair and recombination in phage T4. I. Genes affecting recombination.

7. Bacteriophage T7 morphogenesis: phage-related particles in cells infected with wild-type and mutant T7 phage.

8. EcoRI-sensitive mutation of T7 phage.

9. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

10. Folded, concatenated genomes as replication intermediates of bacteriophage T7 DNA.

1. T7 endonuclease I resolves Holliday junctions formed in vitro by RecA protein.

2. Specificity of binding to four-way junctions in DNA by bacteriophage T7 endonuclease I.

3. Multidimensional analysis of intracellular bacteriophage T7 DNA: effects of amber mutations in genes 3 and 19.

4. Partial purification of an enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions.

5. In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay.

6. Bacteriophage T7 defective in the gene 6 exonuclease promotes site-specific cleavages of T7 DNA in vivo and in vitro.

7. Functional analysis of the ABF1-binding sites within the Ya regions of the MATa and HMRa loci of Saccharomyces cerevisiae.

8. Endonuclease VII resolves Y-junctions in branched DNA in vitro.