Literature DB >> 6274848

Amino-terminal sequence of the Tn3 transposase protein.

M D Ditto, J Chou, M W Hunkapiller, M A Fennewald, S P Gerrard, L E Hood, S N Cohen, M J Casadaban.   

Abstract

The amino-terminal sequence of the Tn3 transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-beta-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-beta-galactosidase gene fusions.

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Year:  1982        PMID： 6274848      PMCID： PMC216644          DOI： 10.1128/jb.149.1.407-410.1982

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

1.  Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product.

Authors:  J Chou; P G Lemaux; M J Casadaban; S N Cohen
Journal:  Nature       Date:  1979 Dec 20-27       Impact factor: 49.962

2.  Identification of the protein encoded by the transposable element Tn3 which is required for its transposition.

Authors:  R E Gill; F Heffron; S Falkow
Journal:  Nature       Date:  1979 Dec 20-27       Impact factor: 49.962

3.  Dissection of the transposition process: a transposon-encoded site-specific recombination system.

Authors:  A Arthur; D Sherratt
Journal:  Mol Gen Genet       Date:  1979-10-01

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.

Authors:  M J Casadaban; J Chou; S N Cohen
Journal:  J Bacteriol       Date:  1980-08       Impact factor: 3.490

6.  Tn3: transposition and control.

Authors:  M J Casadaban; J Chou; P Lemaux; C P Tu; S N Cohen
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1981

7.  Purification of the Tn3 transposase and analysis of its binding to DNA.

Authors:  M A Fennewald; S P Gerrard; J Chou; M J Casadaban; N R Cozzarelli
Journal:  J Biol Chem       Date:  1981-05-25       Impact factor: 5.157

8.  Resolution of cointegrates between transposons gamma delta and Tn3 defines the recombination site.

Authors:  R R Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

9.  New protein sequenator with increased sensitivity.

Authors:  M W Hunkapiller; L E Hood
Journal:  Science       Date:  1980-02-01       Impact factor: 47.728

10.  Localization of the Tn5 transposase promoter using the cycling reaction of RNA polymerase.

Authors:  R C Johnson; W S Reznikoff
Journal:  Nucleic Acids Res       Date:  1981-04-24       Impact factor: 16.971
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  3 in total

1.  Remembering Malcolm J. Casadaban.

Authors: 
Journal:  J Bacteriol       Date:  2010-05-28       Impact factor: 3.490

2.  In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage.

Authors:  M J Casadaban; J Chou
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

3.  Expression of a preproinsulin-beta-galactosidase gene fusion in mammalian cells.

Authors:  D A Nielsen; J Chou; A J MacKrell; M J Casadaban; D F Steiner
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205
  3 in total

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