The histogenesis of so-called mixed tumor evaluating from parotin binding.

We evaluated the histogenesis of so-called mixed tumors of the parotid gland by comparing the tumor cells with normal parotid gland cells. Using the indirect technique of the enzyme antibody method, the paraffin sections of human parotid glands, initially reacted with 0.1 mg/ml of parotin solution in PBS for 20 min at 37 degrees C, were treated for 20 min at 37 degrees C with anti-parotin subunit rabbit IgG (0.01 mg/ml) containing 5 mg/ml BSA, and then with HRPO-labeled anti-rabbit gamma globulin antibodies (swine IgG). After a staining for enzyme by Karnovsky's method, DAB reactions were observed on the duct cells of human parotid glands in 12 of 13 cases. We surveyed the parotin binding as a marker of duct cell on the so-called mixed tumors of 11 cases. DAB reactions were recognized on the cells of myxoid portion, along with the polygonal cells of solid sheet, board cord, and ductular formation, but the cells of chondroid portion and the spindle-shaped cells forming trabecular structure did not.