Literature DB >> 6273981

HPLC analysis of erythrocyte pyrimidine-5'-nucleotidase inhibition by lead.

R M Rocco, J L Smart, D R Brown, J B Blumberg.   

Abstract

The activity of pyrimidine-5'-nucleotidase (P5N) (EC 3.1.3.5) was assayed in microsamples of rat blood using high performance liquid chromotography (HPLC). The assay is based on the measurement of enzymatically formed uridine in erythrocyte hemolysates (10-50 microliter) and produces a linear activity curve from 5 to 1000 microM/g Hb/h. Acute (22 h) administration of lead acetate ip to rats induced a dose-dependent inhibition of P5N activity in erythrocyte samples.

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Year:  1981        PMID： 6273981

Source DB:  PubMed          Journal:  Res Commun Chem Pathol Pharmacol        ISSN： 0034-5164


  3 in total

1.  Erythrocyte arginase, pyrimidine 5'-nucleotidase (P5N), and deoxypyrimidine 5'-nucleotidase (dP5N) as indices of lead exposure.

Authors:  L R Cook; C R Angle; S J Stohs
Journal:  Br J Ind Med       Date:  1986-06

2.  Relationship between inhibition of erythrocyte pyrimidine 5'-nucleotidase activity and biological response for porphyrin metabolism in workers occupationally exposed to lead.

Authors:  K Tomokuni; M Ichiba; Y Hirai; T Hasegawa; K Sugimoto
Journal:  Int Arch Occup Environ Health       Date:  1988       Impact factor: 3.015

3.  Studies on erythrocyte pyrimidine 5'-nucleotidase (P5N) test and its evaluation in workers occupationally exposed to lead.

Authors:  M Ichiba; K Tomokuni
Journal:  Int Arch Occup Environ Health       Date:  1990       Impact factor: 3.015
  3 in total

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