Literature DB >> 6273854

Heteroduplex formation by recA protein: polarity of strand exchanges.

S C West, E Cassuto, P Howard-Flanders.   

Abstract

Purified recA protein promotes strand exchanges between linear duplex DNA and homologous circular single-stranded phage phi X174 DNA that carries a short hybridized fragment [West, S. C., Cassuto, E. & Howard-Flanders, P. (1981) Proc. Natl. Acad. Sci. USA 78, 2100-2104]. In this paper we investigate the mechanism of this strand exchange reaction. We show that recA protein initiates strand exchanges by pairing the free end of the duplex fragment with the single-stranded DNA. In addition, we find that strand exchanges are polar, stable heteroduplex molecules being formed by the directional transfer transfer of the (-) strands starting at 3' termini.

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Year:  1981        PMID： 6273854      PMCID： PMC348995          DOI： 10.1073/pnas.78.10.6149

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

Review 1.  Genetic recombination: strand transfer and mismatch repair.

Authors:  C M Radding
Journal:  Annu Rev Biochem       Date:  1978       Impact factor: 23.643

2.  Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors:  A C Chang; S N Cohen
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

3.  ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli.

Authors:  G M Weinstock; K McEntee; I R Lehman
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

4.  Initiation of recA+-dependent recombination in Escherichia coli (lambda). I. Undamaged covalent circular lambda DNA molecules in uvrA+ recA+ lysogenic host cells are cut following superinfection with psoralen-damaged lambda phages.

Authors:  P Ross; P Howard-Flanders
Journal:  J Mol Biol       Date:  1977-11-25       Impact factor: 5.469

5.  Isolation and characterization of specialized transducing bacteriophages for the recA gene of Escherichia coli.

Authors:  K McEntee; W Estein
Journal:  Virology       Date:  1977-03       Impact factor: 3.616

6.  Susceptibility of the phiX-like phages G4 and G14 to R-EcoRi endonuclease.

Authors:  G N Godson; H Boyer
Journal:  Virology       Date:  1974-11       Impact factor: 3.616

Review 7.  Pedigrees of some mutant strains of Escherichia coli K-12.

Authors:  B J Bachmann
Journal:  Bacteriol Rev       Date:  1972-12

8.  Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.

Authors:  W B Wood
Journal:  J Mol Biol       Date:  1966-03       Impact factor: 5.469

9.  Polarity of heteroduplex formation promoted by Escherichia coli recA protein.

Authors:  R Kahn; R P Cunningham; C DasGupta; C M Radding
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

10.  The nucleotide sequence of bacteriophage phiX174.

Authors:  F Sanger; A R Coulson; T Friedmann; G M Air; B G Barrell; N L Brown; J C Fiddes; C A Hutchison; P M Slocombe; M Smith
Journal:  J Mol Biol       Date:  1978-10-25       Impact factor: 5.469
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  41 in total

1.  Topological testing of the mechanism of homology search promoted by RecA protein.

Authors:  L Cai; U Marquardt; Z Zhang; M J Taisey; J Chen
Journal:  Nucleic Acids Res       Date:  2001-03-15       Impact factor: 16.971

2.  The human Rad51 protein: polarity of strand transfer and stimulation by hRP-A.

Authors:  P Baumann; S C West
Journal:  EMBO J       Date:  1997-09-01       Impact factor: 11.598

3.  Structure and Dynamics of recA Protein-DNA Complexes as Determined by Image Analysis of Electron Micrographs.

Authors:  A Stasiak; E H Egelman
Journal:  Biophys J       Date:  1986-01       Impact factor: 4.033

4.  Base pair switching by interconversion of sugar puckers in DNA extended by proteins of RecA-family: a model for homology search in homologous genetic recombination.

Authors:  T Nishinaka; A Shinohara; Y Ito; S Yokoyama; T Shibata
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-15       Impact factor: 11.205

5.  Branch migration during Rad51-promoted strand exchange proceeds in either direction.

Authors:  E A Namsaraev; P Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205

6.  Presynaptic filament dynamics in homologous recombination and DNA repair.

Authors:  Jie Liu; Kirk T Ehmsen; Wolf-Dietrich Heyer; Scott W Morrical
Journal:  Crit Rev Biochem Mol Biol       Date:  2011-06       Impact factor: 8.250

7.  Gene 4 helicase of bacteriophage T7 mediates strand transfer through pyrimidine dimers, mismatches, and nonhomologous regions.

Authors:  D Kong; J D Griffith; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-01       Impact factor: 11.205

8.  Properties of the duplex DNA-dependent ATPase activity of Escherichia coli RecA protein and its role in branch migration.

Authors:  S C Kowalczykowski; J Clow; R A Krupp
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

9.  Unwinding associated with synapsis of DNA molecules by recA protein.

Authors:  A M Wu; M Bianchi; C DasGupta; C M Radding
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

10.  Polarity of DNA strand exchange promoted by recombination proteins of the RecA family.

Authors:  R C Gupta; E I Golub; M S Wold; C M Radding
Journal:  Proc Natl Acad Sci U S A       Date:  1998-08-18       Impact factor: 11.205
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