| Literature DB >> 6272066 |
M Sawada, S Osawa, H Kobayashi, H Hori, A Muto.
Abstract
We have examined the number of rRNA genes in Mycoplasma capricolum (KID) by hybridization of Bg/II-, EcoRI- and Xbal-digests of DNA to [3'-32P] 16S, 23S and 5S rRNAs according to the Southern procedure (1975). All the restriction gels gave two radioactive bands with three kinds of rRNA. Furthermore, band positions were indistinguishable from one another when 16S, 23S and 5S rRNAs were used as probes, indicating that each band contains sequences corresponding to the 3'-termini of 16S, 23S and 5S rRNAs. It is thus concluded that Mycoplasma capricolum chromosome carries at least two sets of genes for 16S, 23S and 5S rRNAs.Entities:
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Year: 1981 PMID: 6272066 DOI: 10.1007/bf00293942
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925