| Literature DB >> 6269782 |
G P Smith, G D Smith, T J Peters.
Abstract
A highly sensitive fluorimetric assay for pyridoxal phosphate phosphatase is described. The assay involves separation of the substrate and product by ion-exchange chromatography followed by treatment of pyridoxal with potassium cyanide under slightly alkaline conditions to form 4-pyridoxolactone, a highly fluorescent compound. Certain kinetic properties of the enzyme activities in human neutrophils are described.Entities:
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Year: 1981 PMID： 6269782 DOI： 10.1016/0009-8981(81)90398-3
Source DB: PubMed Journal: Clin Chim Acta ISSN： 0009-8981 Impact factor: 3.786