Literature DB >> 6269032

Receptor autoradiography with tritium-sensitive film: potential for computerized densitometry.

J M Palacios, D L Niehoff, M J Kuhar.   

Abstract

Benzodiazepine receptors mounted tissue sections of brain were localized by autoradiography using a tritium-sensitive sheet film (3H-Ultrofilm, LKB). The use of the film had many advantages compared to a previous technique utilizing emulsion-coated coverslips. A major advantage is the adaptability of the film to microdensitometric techniques for measurement of receptors. The densitometry can be carried out by computerized, color-coded image analysis.

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Year:  1981        PMID: 6269032     DOI: 10.1016/0304-3940(81)90315-3

Source DB:  PubMed          Journal:  Neurosci Lett        ISSN: 0304-3940            Impact factor:   3.046


  16 in total

Review 1.  Autoradiographic assessment of the effects of N-methyl-D-aspartate (NMDA) receptor antagonists in vivo.

Authors:  J McCulloch; L L Iversen
Journal:  Neurochem Res       Date:  1991-09       Impact factor: 3.996

2.  A new method for quenching correction leads to revisions of data in receptor autoradiography.

Authors:  K Zilles; K zur Nieden; A Schleicher; J Traber
Journal:  Histochemistry       Date:  1990

Review 3.  Autoradiographical and immunohistochemical analysis of receptor localization in the central nervous system.

Authors:  J G Chabot; S Kar; R Quirion
Journal:  Histochem J       Date:  1996-11

4.  Radioimagers as an alternative to film autoradiography for in situ quantitative analysis of 125I-ligand receptor binding and pharmacological studies.

Authors:  M Crumeyrolle-Arias; M Jafarian-Tehrani; A Cardona; L Edelman; P Roux; P Lanièce; Y Charon; F Haour
Journal:  Histochem J       Date:  1996-11

Review 5.  Regulatory peptide receptors: visualization by autoradiography.

Authors:  J M Palacios; M M Dietl
Journal:  Experientia       Date:  1987-07-15

6.  Autoradiographic localization of "peripheral-type" benzodiazepine binding sites in the rat brain, heart and kidney.

Authors:  D R Gehlert; H I Yamamura; J K Wamsley
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1985-02       Impact factor: 3.000

Review 7.  Benzodiazepine receptors resolved.

Authors:  J G Richards; P Schoch; H Möhler; W Haefely
Journal:  Experientia       Date:  1986-02-15

8.  Kappa opiate receptors localized by autoradiography to deep layers of cerebral cortex: relation to sedative effects.

Authors:  R R Goodman; S H Snyder
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

9.  Muscarinic receptors in the prenatal mouse embryo. Comparison of M35-immunohistochemistry with [3H]quinuclidinyl benzylate autoradiography.

Authors:  M Lammerding-Köppel; A Greiner-Schröder; U Drews
Journal:  Histochem Cell Biol       Date:  1995-04       Impact factor: 4.304

10.  Compartmentalization of excitatory amino acid receptors in human striatum.

Authors:  L S Dure; A B Young; J B Penney
Journal:  Proc Natl Acad Sci U S A       Date:  1992-08-15       Impact factor: 11.205

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