Literature DB >> 6268725

A new solvent system for the separation of neutral glycosphingolipids.

K Watanabe, Y Arao.   

Abstract

A solvent system and a column for high performance liquid chromatography for the separation of glycosphingolipids without derivatization is described. A column pakced with porous silica gel (latrobeads) and eluted with a mixture of isopropanol-hexane-water with increasing water content and decreasing hexane content was used. Glycosphingolipids with mono- to dodeca- or tetrakaidecasaccharides were separated within 60 min and the separation pattern was highly reproducible. The method was applied for preparative separation of highly complex glycolipids with blood group activity.

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Year:  1981        PMID： 6268725

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  17 in total

1.  Serum immunoassay of a small cell lung cancer associated ganglioside: development of a sensitive scintillation proximity assay.

Authors:  T Pallesen; A Vangsted; L Drivsholm; H Clausen; J Zeuthen; H Wallin
Journal:  Glycoconj J       Date:  1992-12       Impact factor: 2.916

2.  Separation of underivatized gangliosides by ion exchange high performance liquid chromatography.

Authors:  M M Whalen; G C Wild; W D Spall; R J Sebring
Journal:  Lipids       Date:  1986-04       Impact factor: 1.880

3.  Possible role of ceramide in defining structure and function of membrane glycolipids.

Authors:  R Kannagi; E Nudelman; S Hakomori
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

4.  Molecular species analysis of glycosphingolipids from small intestine of Japanese quail, Coturnix coturnix Japonica by HPLC/FAB/MS.

Authors:  A C Suzuki; A Nakamura; K Nishimura
Journal:  Glycoconj J       Date:  1994-04       Impact factor: 2.916

5.  Sequential change of carbohydrate antigen associated with differentiation of murine leukemia cells: i-I antigenic conversion and shifting of glycolipid synthesis.

Authors:  R Kannagi; S B Levery; S Hakomori
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205

6.  Structural studies of gangliosides from the YAC-1 mouse lymphoma cell line by immunological detection and fast atom bombardment mass spectrometry.

Authors:  J Müthing; J Peter-Katalinić; F G Hanisch; U Neumann
Journal:  Glycoconj J       Date:  1991-10       Impact factor: 2.916

7.  Structural studies on the neutral glycosphingolipids of Manduca sexta.

Authors:  D Thusitha U Abeytunga; Lynne Oland; Arpad Somogyi; Robin Polt
Journal:  Bioorg Chem       Date:  2007-11-19       Impact factor: 5.275

8.  Monoclonal antibody reveals heterogeneity in human aortic intima: detection of a ganglioside antigen associated with a subpopulation of intimal cells.

Authors:  A N Orekhov; G F Kalantarov; E R Andreeva; N V Prokazova; I N Trakht; L D Bergelson; V N Smirnov
Journal:  Am J Pathol       Date:  1986-03       Impact factor: 4.307

9.  The globoseries glycosphingolipid sialosyl galactosyl globoside is found in urinary tract tissues and is a preferred binding receptor In vitro for uropathogenic Escherichia coli expressing pap-encoded adhesins.

Authors:  A E Stapleton; M R Stroud; S I Hakomori; W E Stamm
Journal:  Infect Immun       Date:  1998-08       Impact factor: 3.441

10.  Normally occurring human anti-GM1 immunoglobulin M antibodies and the immune response to bacteria.

Authors:  María E Alaniz; Ricardo D Lardone; Silvia L Yudowski; María I Farace; Gustavo A Nores
Journal:  Infect Immun       Date:  2004-04       Impact factor: 3.441
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