Literature DB >> 6267543

Separation of Isospora (Toxoplasma) gondii cysts and cystozoites from mouse brain tissue by continuous density-gradient centrifugation.

A W Cornelissen, J P Overdulve, J M Hoenderboom.   

Abstract

A simple, quick and reproducible method consisting of density-gradient centrifugation of homogenized infected mouse brain tissue on Percoll is described for the isolation and purification of cysts of Isospora (Toxoplasma) gondii. A 100% recovery of cysts, with 74.2% in a single fraction with a specific gravity of 1.056, was obtained by overlaying homogenates of infected mouse brains on a pre-formed Percoll gradient and centrifugation at low g forces. With this procedure recovery was independent of the age of the cysts. Titration of purified cystozoites showed there to be no loss of infectivity.

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Year:  1981        PMID： 6267543     DOI： 10.1017/s0031182000050071

Source DB:  PubMed          Journal:  Parasitology        ISSN： 0031-1820            Impact factor:   3.234


  39 in total

Review 1.  The development and biology of bradyzoites of Toxoplasma gondii.

Authors:  L M Weiss; K Kim
Journal:  Front Biosci       Date:  2000-04-01

2.  Disruption of the Toxoplasma gondii bradyzoite-specific gene BAG1 decreases in vivo cyst formation.

Authors:  Y W Zhang; K Kim; Y F Ma; M Wittner; H B Tanowitz; L M Weiss
Journal:  Mol Microbiol       Date:  1999-01       Impact factor: 3.501

3.  Activity of the histone deacetylase inhibitor FR235222 on Toxoplasma gondii: inhibition of stage conversion of the parasite cyst form and study of new derivative compounds.

Authors:  Danièle Maubon; Alexandre Bougdour; Yung-Sing Wong; Marie-Pierre Brenier-Pinchart; Aurélie Curt; Mohamed-Ali Hakimi; Hervé Pelloux
Journal:  Antimicrob Agents Chemother       Date:  2010-08-16       Impact factor: 5.191

4.  Characterization of bradyzoite-specific antigens of Toxoplasma gondii.

Authors:  S Tomavo; B Fortier; M Soete; C Ansel; D Camus; J F Dubremetz
Journal:  Infect Immun       Date:  1991-10       Impact factor: 3.441

5.  Reexamining Chronic Toxoplasma gondii Infection: Surprising Activity for a "Dormant" Parasite.

Authors:  Anthony P Sinai; Elizabeth A Watts; Animesh Dhara; Robert D Murphy; Matthew S Gentry; Abhijit Patwardhan
Journal:  Curr Clin Microbiol Rep       Date:  2016-10-04

6.  Parasitology as a teaching tool: isolation of apicomplexan cysts from store-bought meat.

Authors:  Tracy L Eggleston; Eileen Fitzpatrick; Kristin M Hager
Journal:  CBE Life Sci Educ       Date:  2008       Impact factor: 3.325

7.  Modulation of early β-defensin-2 production as a mechanism developed by type I Toxoplasma gondii to evade human intestinal immunity.

Authors:  Vijay Morampudi; Michel Y Braun; Sushila D'Souza
Journal:  Infect Immun       Date:  2011-03-07       Impact factor: 3.441

8.  Detection of nucleoside triphosphate hydrolase as a circulating antigen in sera of mice infected with Toxoplasma gondii.

Authors:  T Asai; T J Kim; M Kobayashi; S Kojima
Journal:  Infect Immun       Date:  1987-05       Impact factor: 3.441

9.  Purification Toxoplasma gondii Tissue Cysts Using Percoll Gradients.

Authors:  Elizabeth A Watts; Animesh Dhara; Anthony P Sinai
Journal:  Curr Protoc Microbiol       Date:  2017-05-16

10.  Interleukin 12 is required for the T-lymphocyte-independent induction of interferon gamma by an intracellular parasite and induces resistance in T-cell-deficient hosts.

Authors:  R T Gazzinelli; S Hieny; T A Wynn; S Wolf; A Sher
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-01       Impact factor: 11.205
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