Literature DB >> 6267435

DNA replication intermediates synthesized by lysates of dnaB, dnaG and dnaB dnaG mutants in vitro.

R A Sclafani, J A Wechsler.   

Abstract

Isogenic dnaB, dnaG, and dnaB dnaG mutants were constructed and used as extracts in the cellophane-disc in vitro DNA replication system. The increased proportion of 5S DNA characteristics of the dnab extract and the lack of Okazaki piece synthesis characteristic of the dnaG extract were both apparent in analysis of the dnaB dnaG mutant extract reaction. A hypothetical scheme to explain these results and those of others is presented.

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Year:  1981        PMID： 6267435     DOI： 10.1007/bf00422773

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  23 in total

1.  Genetic map location of the Escherichia coli dnaG gene.

Authors:  P L Chen; P L Carl
Journal:  J Bacteriol       Date:  1975-12       Impact factor: 3.490

2.  dnaB protein of Escherichia coli. Purification and role in the replication of phiX174 DNA.

Authors:  K Ueda; R McMacken; A Kornberg
Journal:  J Biol Chem       Date:  1978-01-10       Impact factor: 5.157

3.  Enzyme-catalyzed DNA unwinding: studies on Escherichia coli rep protein.

Authors:  G T Yarranton; M L Gefter
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

4.  DNA replication proteins of Escherichia coli and phage lambda.

Authors:  S H Wickner
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

5.  Different arrangements of protein subunits and single-stranded circular DNA in the filamentous bacterial viruses fd and Pf1.

Authors:  R L Wiseman; S A Berkowitz; L A Day
Journal:  J Mol Biol       Date:  1976-04-15       Impact factor: 5.469

6.  Deoxyribonucleic acid replication in vitro.

Authors:  H Schaller; B Otto; V Nüsslein; J Huf; R Herrmann; F Bonhoeffer
Journal:  J Mol Biol       Date:  1972-01-28       Impact factor: 5.469

7.  Genetic control over the initiation of the synthesis of the short deoxynucleotide chains in E. coli.

Authors:  K G Lark
Journal:  Nat New Biol       Date:  1972-12-20

8.  The tolC locus in Escherichia coli K12.

Authors:  E N Whitney
Journal:  Genetics       Date:  1971-01       Impact factor: 4.562

9.  dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form.

Authors:  J P Bouché; K Zechel; A Kornberg
Journal:  J Biol Chem       Date:  1975-08-10       Impact factor: 5.157

10.  Efficient in vitro replication of double-stranded DNA templates by a purified T4 bacteriophage replication system.

Authors:  N K Sinha; C F Morris; B M Alberts
Journal:  J Biol Chem       Date:  1980-05-10       Impact factor: 5.157
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  1 in total

1.  Deletion map of the Escherichia coli K-12 dnaB gene.

Authors:  R A Sclafani; J A Wechsler
Journal:  Mol Gen Genet       Date:  1981
  1 in total

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