Literature DB >> 6267425

Introduction of active enzymes into intact Escherichia coli cells by means of liposomes. Phenotypic suppression of uvr A and pol A mutants.

S E Bresler, L A Noskin, O K Kaboev, I M Stepanova, E F Davidenkova, O A Rosenberg, E I Schwartz, A G Beketova, A T Akhmedov.   

Abstract

Genetically deficient cells were supplied with the missing enzymes, purified from an independent source. The introduction of exogenous enzymes into the cells was effected by two independent methods: plasmolysis and liposome transformation. The latter procedure yielded a homogenous cell population which had been rescued from the defect even if the molecular weight of the enzyme amounted to 70 KD (Kilodaltons).

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Year:  1981        PMID: 6267425     DOI: 10.1007/bf00428748

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN: 0026-8925


  15 in total

1.  CHROMATOGRAPHICALLY HOMOGENEOUS LECITHIN FROM EGG PHOSPHOLIPIDS.

Authors:  W S SINGLETON; M S GRAY; M L BROWN; J L WHITE
Journal:  J Am Oil Chem Soc       Date:  1965-01       Impact factor: 1.849

2.  QUANTITATIVE TWO-DIMENSIONAL THIN-LAYER CHROMATOGRAPHY OF NATURALLY OCCURRING PHOSPHOLIPIDS.

Authors:  D ABRAMSON; M BLECHER
Journal:  J Lipid Res       Date:  1964-10       Impact factor: 5.922

3.  Light scattering and turbidity measurements on lipid vesicles.

Authors:  C S Chong; K Colbow
Journal:  Biochim Biophys Acta       Date:  1976-06-17

4.  Further purification and characterization of T4 endonuclease V.

Authors:  S Yasuda; M Sekiguchi
Journal:  Biochim Biophys Acta       Date:  1976-08-18

5.  Introduction of an active enzyme into permeable cells of Escherichia coli: acquisition of ultraviolet light resistance by uvr mutants on introduction of T4 endonuclease V.

Authors:  K Shimizu; M Sekiguchi
Journal:  Mol Gen Genet       Date:  1979-01-05

6.  Ultraviolet-endonuclease activity in cell extracts of Saccharomyces cerevisiae mutants defective in excision of pyrimidine dimers.

Authors:  M L Bekker; O K Kaboev; A T Akhmedov; L A Luchkina
Journal:  J Bacteriol       Date:  1980-04       Impact factor: 3.490

7.  A universal reagent for phospholipid analysis.

Authors:  V E Vaskovsky; E Y Kostetsky; I M Vasendin
Journal:  J Chromatogr       Date:  1975-11-12

8.  Lipid vesicles as carriers for introducing materials into cultured cells: influence of vesicle lipid composition on mechanism(s) of vesicle incorporation into cells.

Authors:  G Poste; D Papahadjopoulos
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

9.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

10.  Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation.

Authors:  F Szoka; D Papahadjopoulos
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

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