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Literature DB >> 6265868

Characterization of a cloned histone gene cluster of the newt Notophthalamus viridescens.

Abstract

We report the cloning and characterization of a histone gene cluster of the newt Notophthalamus viridescens. Fragments containing newt histone genes were identified in whole genome Southern blots; these fragments were cloned into a bacteriophage lambda cloning vector constructed for this purpose. The positions of most of the histone genes were determined by hybridizing subcloned sea urchin histone genes to digests of the cloned newt gene cluster. The position of each gene was verified, and its polarity determined by sequencing a portion of each. The order of the genes in the cloned segment is H1-H3-H2B-H2A-H4, with each of the genes but H2B being transcribed in the same direction. Subcloned segments of the histone gene repeat were used to determine the size of each newt oocyte histone mRNA.

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Year: 1981 PMID： 6265868 PMCID： PMC326845 DOI： 10.1093/nar/9.10.2281

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

36 in total

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors: T Maniatis; A Jeffrey; H van deSande
Journal: Biochemistry Date: 1975-08-26 Impact factor: 3.162

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. Histone 3. 3. Sequence studies on the cyanogen bromide peptides; complete amino acid sequence of calf thymus histone 3.

Authors: R J DeLange; J A Hooper; E L Smith
Journal: J Biol Chem Date: 1973-05-10 Impact factor: 5.157

4. Covalent structure of calf-thymus ALK-histone.

Authors: P Sautière; D Tyrou; B Laine; J Mizon; P Ruffin; G Biserte
Journal: Eur J Biochem Date: 1974-02-01

5. Chromosome-sized DNA molecules from Drosophila.

Authors: R Kavenoff; B H Zimm
Journal: Chromosoma Date: 1973 Impact factor: 4.316

6. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

7. Molecular analysis of the histone gene cluster of Psammechinus miliaris: III. Polarity and asymmetry of the histone-coding sequences.

Authors: K Gross; W Schaffner; J Telford; M Birnstiel
Journal: Cell Date: 1976-08 Impact factor: 41.582

Characterization of a cloned histone gene cluster of the newt Notophthalamus viridescens.

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

3. Histone 3. 3. Sequence studies on the cyanogen bromide peptides; complete amino acid sequence of calf thymus histone 3.

4. Covalent structure of calf-thymus ALK-histone.

5. Chromosome-sized DNA molecules from Drosophila.

6. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

7. Molecular analysis of the histone gene cluster of Psammechinus miliaris: III. Polarity and asymmetry of the histone-coding sequences.

8. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

9. Nucleotide sequence of the rightward operator of phage lambda.

10. Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA.

1. A comprehensive compilation and alignment of histones and histone genes.

2. Microdissection and cloning of DNA from landmark loops of amphibian lampbrush chromosomes.

3. Subfamilies of histone H3 and H4 genes are located on most, possibly all of the chromosomes in maize.

4. Cloning and characterization of a core histone gene tandem repeat in Urechis caupo.

5. DNA-binding proteins on lampbrush chromosome loops.

6. Giant readthrough transcription units at the histone loci on lampbrush chromosomes of the newt Notophthalmus.

7. Transcription of the histone loci on lampbrush chromosomes of the newt Notophthalmus viridescens.

8. Compilation analysis of histones and histone genes.

9. Organization and expression of cloned histone gene clusters from Xenopus laevis and X. borealis.

10. Histone gene number and organisation in Xenopus: Xenopus borealis has a homogeneous major cluster.