Literature DB >> 6261684

Extraction of rotavirus from human feces by treatment with lithium dodecyl sulfate.

M C Croxson, A R Bellamy.   

Abstract

A procedure has been developed for the isolation of rotavirus from human fecal specimens based on the resistance of the virus to treatment with cold 1% lithium dodecyl sulfate at neutral pH. A single detergent treatment of fecal material followed by low- and high-speed centrifugations yielded a virus suspension of sufficient purity for viral ribonucleic acid to be analyzed directly by electrophoresis on polyacrylamide gels.

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Year:  1981        PMID： 6261684      PMCID： PMC243673          DOI： 10.1128/aem.41.1.255-260.1981

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  22 in total

1.  Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis.

Authors:  R F Bishop; G P Davidson; I H Holmes; B J Ruck
Journal:  Lancet       Date:  1973-12-08       Impact factor: 79.321

2.  Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis.

Authors:  R F Bishop; G P Davidson; I H Holmes; B J Ruck
Journal:  Lancet       Date:  1974-02-02       Impact factor: 79.321

3.  In vitro assembly of poliovirus-related particles.

Authors:  B A Phillips; D F Summers; J V Maizel
Journal:  Virology       Date:  1968-06       Impact factor: 3.616

4.  RNA polymerase activity in purified reoviruses.

Authors:  A J Shatkin; J D Sipe
Journal:  Proc Natl Acad Sci U S A       Date:  1968-12       Impact factor: 11.205

5.  Analysis of bacteriophage T7 early RNAs and proteins on slab gels.

Authors:  F W Studier
Journal:  J Mol Biol       Date:  1973-09-15       Impact factor: 5.469

6.  Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus cores.

Authors:  J J Skehel; W K Joklik
Journal:  Virology       Date:  1969-12       Impact factor: 3.616

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Reovirus: RNA polymerase activity in purified virions.

Authors:  J Borsa; A F Graham
Journal:  Biochem Biophys Res Commun       Date:  1968-12-30       Impact factor: 3.575

9.  Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin.

Authors:  S M Rodger; R D Schnagl; I H Holmes
Journal:  J Virol       Date:  1975-11       Impact factor: 5.103

10.  The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.

Authors:  A E Bolton; W M Hunter
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857
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  4 in total

1.  Occurrence of nonspecific reactions among stool specimens tested by the Abbott TestPack rotavirus enzyme immunoassay.

Authors:  S M Lipson; G P Leonardi; R J Salo; T E Schutzbank; M H Kaplan
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

2.  Genetic heterogeneity within individual bovine rotavirus isolates.

Authors:  M Sabara; D Deregt; L A Babiuk; V Misra
Journal:  J Virol       Date:  1982-12       Impact factor: 5.103

3.  Sequence diversity of human rotavirus strains investigated by northern blot hybridization analysis.

Authors:  J E Street; M C Croxson; W F Chadderton; A R Bellamy
Journal:  J Virol       Date:  1982-08       Impact factor: 5.103

4.  Sequence relationships between the genome segments of human and animal rotavirus strains.

Authors:  B A Schroeder; J E Street; J Kalmakoff; A R Bellamy
Journal:  J Virol       Date:  1982-08       Impact factor: 5.103
  4 in total

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