Literature DB >> 6261107

Detection of IgG antibodies against Bordetella pertussis with 125I-protein A.

C H von König, H Finger.   

Abstract

A method for the detection of IgG antibodies against Bordetella pertussis is described, based on the principle of 'sandwich' radioimmunoassay. 125I protein A is used as radioactive tracer. The influence of amounts of antigen, antibody, radioactive tracer, incubation time and temperature were tested and the optimal conditions for the assay are described. The procedure offers a simple, quick, and sensitive method for detecting antibodies against B. pertussis. Application and limitation of the test are discussed.

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Year:  1981        PMID： 6261107     DOI： 10.1007/bf02171775

Source DB:  PubMed          Journal:  Med Microbiol Immunol        ISSN： 0300-8584            Impact factor:   3.402


  7 in total

1.  Structure and immunology of protein A.

Authors:  J Sjöquist
Journal:  Contrib Microbiol Immunol       Date:  1973

2.  [125I]protein A: a tracer for general use in immunoassay.

Authors:  J J Langone
Journal:  J Immunol Methods       Date:  1978       Impact factor: 2.303

3.  Differences in anti-protein A activity among IgG subgroups.

Authors:  G Kronvall; R C Williams
Journal:  J Immunol       Date:  1969-10       Impact factor: 5.422

4.  Rapid diagnosis of Pseudomonas aeruginosa urinary tract infections by radioimmunoassay.

Authors:  R B Kohler; L J Wheat; A White
Journal:  J Clin Microbiol       Date:  1979-02       Impact factor: 5.948

5.  The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.

Authors:  A E Bolton; W M Hunter
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857

6.  Immune response in urinary tract infection determined by radioimmunoassay and immunofluorescence: serum antibody levels against infecting bacterium and Enterobacteriaceae common antigen.

Authors:  B A Sanford; V L Thomas; M Forland; S Carson; A Shelokov
Journal:  J Clin Microbiol       Date:  1978-11       Impact factor: 5.948

7.  Solid phase radioimmunoassay for quantitation of antigen-specific IgG in human sera with 125I-protein A from Staphylococcus aureus.

Authors:  R G Hamilton; A K Sobotka; N F Adkinson
Journal:  J Immunol       Date:  1979-03       Impact factor: 5.422
  7 in total
  1 in total

1.  Comparison of five commercial enzyme-linked immunosorbent assays for detection of antibodies to Bordetella pertussis.

Authors:  K Kösters; M Riffelmann; B Dohrn; C H von König
Journal:  Clin Diagn Lab Immunol       Date:  2000-05
  1 in total

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