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Literature DB >> 6261019

Cloning of restriction fragments of DNA from staphylococcal bacteriophage phi 11.

Abstract

EcoRI fragments of Staphylococcus aureus bacteriophage phi 11 DNA were cloned in vector plasmid pSA2100 in S. aureus. The clones were analyzed in marker rescue experiments with suppressor- and temperature-sensitive mutants of phi 11 to correlate the genetic and physical map. Several mutants could be identified on the physical map, and a clone containing fragment EcoRI-B of phi 11 DNA expressed immunity to phage infection. In addition, it was found that recombinant plasmids containing phi 11 DNA sequences can be transferred by high-frequency transduction after phage phi 11 infection of host cells.

Entities: Disease Species

Mesh：

Substances：

Year: 1981 PMID： 6261019 PMCID： PMC171067

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

15 in total

1. Recombinant plasmid obtained from two different, compatible staphylococcal plasmids.

Authors: S Iordănescu
Journal: J Bacteriol Date: 1975-11 Impact factor: 3.490

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus.

Authors: R Novick
Journal: Virology Date: 1967-09 Impact factor: 3.616

4. Morphology and physical properties of Staphylococcus bacteriophage P11-M15.

Authors: D T Brown; N C Brown; B T Burlingham
Journal: J Virol Date: 1972-04 Impact factor: 5.103

5. Competence for transfection in Staphylococcus aureus.

Authors: J E Sjöström; M Lindberg; L Philipson
Journal: J Bacteriol Date: 1973-02 Impact factor: 3.490

6. Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes.

Authors: T Mattson; G Van Houwe; A Bolle; G Selzer; R Epstein
Journal: Mol Gen Genet Date: 1977-09-09

10. Mutations in prophage phi11 that impair the transducibility of their Staphylococcus aureus lysogens for methicillin resistance.

Authors: S Cohen; H M Sweeney; S K Basu
Journal: J Bacteriol Date: 1977-01 Impact factor: 3.490

15 in total

10. Cloning, sequencing, and genetic characterization of regulatory genes, rinA and rinB, required for the activation of staphylococcal phage phi 11 int expression.

Authors: Z H Ye; C Y Lee
Journal: J Bacteriol Date: 1993-02 Impact factor: 3.490

Cloning of restriction fragments of DNA from staphylococcal bacteriophage phi 11.

1. Recombinant plasmid obtained from two different, compatible staphylococcal plasmids.

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

3. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus.

4. Morphology and physical properties of Staphylococcus bacteriophage P11-M15.

5. Competence for transfection in Staphylococcus aureus.

6. Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes.

7. Genetic map of the Staphylococcal bacteriophage phi11.

8. Role of the phi 11 phage genome in competence of Staphylococcus aureus.

9. Transformation in Staphylococcus aureus: role of bacteriophage and incidence of competence among strains.

10. Mutations in prophage phi11 that impair the transducibility of their Staphylococcus aureus lysogens for methicillin resistance.

1. Extent of the DNA sequence required in integration of staphylococcal bacteriophage L54a.

Review 2. Recombination-dependent concatemeric plasmid replication.

3. Sequence analysis and comparison of int and xis genes from staphylococcal bacteriophages L54a and phi 11.

4. The generation of concatemeric plasmid DNA in Bacillus subtilis as a consequence of bacteriophage SPP1 infection.

5. Generation of transducing particles in Staphylococcus aureus.

6. Structural analysis of staphylococcal bacteriophage phi 11 attachment sites.

7. Rapid isolation of DNA from Staphylococcus aureus.

8. Autogenous transduction of phi 11de in Staphylococcus aureus: transfer and genetic properties.

9. Structure and restriction enzyme maps of the circularly permuted DNA of staphylococcal bacteriophage phi 11.

10. Cloning, sequencing, and genetic characterization of regulatory genes, rinA and rinB, required for the activation of staphylococcal phage phi 11 int expression.