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Literature DB >> 6260978

Mapping of the viral mRNA encoding a super-T antigen of 115,000 daltons expressed in simian virus 40-transformed rat cell lines.

E May, M Kress, L Daya-Grosjean, R Monier, P May.

Abstract

Simian virus 40-transformed V11 F1 clone 1 subclone 7 rat cells produced a considerable amount of an elongated form of large-T antigen with an Mr of 115,000 (115K super-T antigen), but these cells did not produce detectable traces of normal-sized large-T antigen (86,000 daltons) (P. May, M. Kress, M. Lange, and E. May, Cold Spring Harbor Symp. Quant. Biol. 44:189-200, 1980). First, a comparison of the tryptic peptide fingerprints of 115K super-T and large-T antigens suggested that 115K super-T antigen is simian virus 40 coded and contains a duplication of amino acid sequences of large-T antigen. Second, from S1 mapping analysis of 115K super-T mRNA, performed with various restriction fragments of simian virus 40 DNA, it was concluded that super-T mRNA is a form of large-T mRNA containing a tandem duplication of the sequence extending from approximately 0.46 to 0.35 map unit. The duplicated sequence corresponded to that region of the simian virus 40 genome in which 12 of 13 tsA mutation sites are clustered (C. J. Lai and D. Nathans, Virology 66:70-81, 1975).

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Year: 1981 PMID： 6260978 PMCID： PMC170978

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

24 in total

1. A map of temperature-sensitive mutants of simian virus 40.

Authors: C J Lai; D Nathans
Journal: Virology Date: 1975-07 Impact factor: 3.616

2. Cellular and cell-free synthesis of simian virus 40 T-antigens in permissive and transformed cells.

Authors: C Prives; Y Gluzman; E Winocour
Journal: J Virol Date: 1978-02 Impact factor: 5.103

3. Spliced early mRNAs of simian virus 40.

Authors: A J Berk; P A Sharp
Journal: Proc Natl Acad Sci U S A Date: 1978-03 Impact factor: 11.205

4. The genome of simian virus 40.

Authors: V B Reddy; B Thimmappaya; R Dhar; K N Subramanian; B S Zain; J Pan; P K Ghosh; M L Celma; S M Weissman
Journal: Science Date: 1978-05-05 Impact factor: 47.728

5. Nucleotide sequence of the simian virus 40 small-t gene.

Authors: G Volckaert; A Van de Voorde; W Fiers
Journal: Proc Natl Acad Sci U S A Date: 1978-05 Impact factor: 11.205

6. Complete nucleotide sequence of SV40 DNA.

Authors: W Fiers; R Contreras; G Haegemann; R Rogiers; A Van de Voorde; H Van Heuverswyn; J Van Herreweghe; G Volckaert; M Ysebaert
Journal: Nature Date: 1978-05-11 Impact factor: 49.962

7. Gel electrophoretic separation of the complementary strands of bacteriophage DNA.

Authors: G S Hayward
Journal: Virology Date: 1972-07 Impact factor: 3.616

8. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Authors: M W McDonell; M N Simon; F W Studier
Journal: J Mol Biol Date: 1977-02-15 Impact factor: 5.469

9. An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors: H R Pelham; R J Jackson
Journal: Eur J Biochem Date: 1976-08-01

10. Isolation and characterization of poly(A)-containing polyoma "early" and "late" messenger RNAs.

Authors: L J Rosenthal
Journal: Nucleic Acids Res Date: 1976-03 Impact factor: 16.971

17 in total

1. Origin binding by a 100,000-dalton super-T antigen from SVT2 cells.

Authors: V G Wilson; R L Williams
Journal: J Virol Date: 1985-10 Impact factor: 5.103

2. Evidence of transcription from the late region of the integrated simian virus 40 genome in transformed cells: location of the 5' ends of late transcripts in cells abortively infected and in cells transformed by simian virus 40.

Authors: M Ernoult-Lange; E May
Journal: J Virol Date: 1983-06 Impact factor: 5.103

10. Nonlytic simian virus 40-specific 100K phosphoprotein is associated with anchorage-independent growth in simian virus 40-transformed and revertant mouse cell lines.

Authors: S Chen; M Verderame; A Lo; R Pollack
Journal: Mol Cell Biol Date: 1981-11 Impact factor: 4.272

Mapping of the viral mRNA encoding a super-T antigen of 115,000 daltons expressed in simian virus 40-transformed rat cell lines.

1. A map of temperature-sensitive mutants of simian virus 40.

2. Cellular and cell-free synthesis of simian virus 40 T-antigens in permissive and transformed cells.

3. Spliced early mRNAs of simian virus 40.

4. The genome of simian virus 40.

5. Nucleotide sequence of the simian virus 40 small-t gene.

6. Complete nucleotide sequence of SV40 DNA.

7. Gel electrophoretic separation of the complementary strands of bacteriophage DNA.

8. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

9. An efficient mRNA-dependent translation system from reticulocyte lysates.

10. Isolation and characterization of poly(A)-containing polyoma "early" and "late" messenger RNAs.

1. Origin binding by a 100,000-dalton super-T antigen from SVT2 cells.

2. Evidence of transcription from the late region of the integrated simian virus 40 genome in transformed cells: location of the 5' ends of late transcripts in cells abortively infected and in cells transformed by simian virus 40.

3. Characterization of a gene encoding a 115 K super T antigen expressed by a SV40-transformed rat cell line.

4. Functional analysis of a simian virus 40 super T-antigen.

5. Structure and synthesis of a simian virus 40 super T-antigen.

6. Reacquisition of a functional early region by a mouse transformant containing only defective simian virus 40 DNA.

7. New insights into the mechanism of inhibition of p53 by simian virus 40 large T antigen.

8. Structure and biochemical functions of four simian virus 40 truncated large-T antigens.

9. Phosphorylation pattern of large T antigens in mouse cells infected by simian virus 40 wild type or deletion mutants.

10. Nonlytic simian virus 40-specific 100K phosphoprotein is associated with anchorage-independent growth in simian virus 40-transformed and revertant mouse cell lines.