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Literature DB >> 6257655

Characterization of a site-specific restriction endonuclease from Streptomyces aureofaciens.

J Timko, A H Horwitz, J Zelinka, G Wilcox.

Abstract

A new type II sequence-specific restriction endonuclease, SauI, was isolated from Streptomyces aureofaciens IKA18/4. The purified enzyme was free of contaminating exonuclease and phosphatase activities. SauI cleaved lambda DNA at two sites, but did not cleave pBR322, simian virus 40, or phi X174 DNA. SauI recognized the septanucleotide sequence 5'-CCTNAGG-3' and cleaved at the position indicated by the arrow, producing a trinucleotide 5'-terminal extension.

Entities: Chemical Species

Mesh：

Substances：
DNA Restriction Enzymes

Year: 1981 PMID： 6257655 PMCID： PMC217192 DOI： 10.1128/jb.145.2.873-877.1981

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

14 in total

Review 1. Directory of restriction endonucleases.

Authors: R J Roberts
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

2. A thermostable sequence-specific endonuclease from Thermus aquaticus.

Authors: S Sato; C A Hutchinson; J I Harris
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

3. 'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases.

Authors: R W Blakesley; R D Wells
Journal: Nature Date: 1975-10-02 Impact factor: 49.962

4. A new restriction endonuclease from the anaerobic bacterium, Desulfovibrio desulfuricans, Norway.

Authors: R A Makula; R B Meagher
Journal: Nucleic Acids Res Date: 1980-07-25 Impact factor: 16.971

5. The use of thin acrylamide gels for DNA sequencing.

Authors: F Sanger; A R Coulson
Journal: FEBS Lett Date: 1978-03-01 Impact factor: 4.124

6. New map of bacteriophage lambda DNA.

Authors: D L Daniels; J R de Wet; F R Blattner
Journal: J Virol Date: 1980-01 Impact factor: 5.103

7. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

8. DNA sequence of the araC regulatory gene from Escherichia coli B/r.

Authors: C G Miyada; A H Horwitz; L G Cass; J Timko; G Wilcox
Journal: Nucleic Acids Res Date: 1980-11-25 Impact factor: 16.971

9. Specific endonucleases in Streptomyces aureofaciens.

Authors: J Timko; M Matusková; E Zelinková; J Zelinka
Journal: Folia Microbiol (Praha) Date: 1978 Impact factor: 2.099

10. Studies on polynucleotides. LXIX. Synthetic deoxyribopolynucleotides as templates for the DNA polymerase of Escherichia coli: DNA-like polymers containing repeating trinucleotide sequences.

Authors: R D Wells; T M Jacob; S A Narang; H G Khorana
Journal: J Mol Biol Date: 1967-07-28 Impact factor: 5.469

7 in total

Characterization of a site-specific restriction endonuclease from Streptomyces aureofaciens.

Review 1. Directory of restriction endonucleases.

2. A thermostable sequence-specific endonuclease from Thermus aquaticus.

3. 'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases.

4. A new restriction endonuclease from the anaerobic bacterium, Desulfovibrio desulfuricans, Norway.

5. The use of thin acrylamide gels for DNA sequencing.

6. New map of bacteriophage lambda DNA.

7. Sequencing end-labeled DNA with base-specific chemical cleavages.

8. DNA sequence of the araC regulatory gene from Escherichia coli B/r.

9. Specific endonucleases in Streptomyces aureofaciens.

10. Studies on polynucleotides. LXIX. Synthetic deoxyribopolynucleotides as templates for the DNA polymerase of Escherichia coli: DNA-like polymers containing repeating trinucleotide sequences.

1. Restriction enzymes and their isoschizomers.

2. Restriction enzymes and their isoschizomers.

3. Two new restriction endonucleases DraII and DraIII from Deinococcus radiophilus.

4. Restriction and modification enzymes and their recognition sequences.

5. Restriction enzymes and their isoschizomers.

6. Restriction and modification enzymes and their recognition sequences.

7. Restriction and modification enzymes and their recognition sequences.