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Literature DB >> 6256759

Immunoreactive helix-destabilizing protein localized in transcriptionally active regions of Drosophila polytene chromosomes.

Abstract

A highly purified helix-destabilizing protein (HDP) obtained from rat liver has been used to elicit specific, high-titer anti-HDP sera in rabbits. These antisera show immunological crossreaction with single-stranded DNA binding proteins from several very diverse eukaryotic sources, including Drosophila embryos. The use of such antisera in the labeling of Drosophila salivary gland chromosomes by indirect immunofluorescence shows concentrations of immunoreactive HDP in many regions, but especially in chromosome puffs. There is a striking localization of HDP in heat shock puffs known to be sites of new transcription. The pattern of HDP distribution seems to implicate a transcriptional role, with some specificities independent of puffing itself.

Entities: Disease Gene Species

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Year: 1980 PMID： 6256759 PMCID： PMC350366 DOI： 10.1073/pnas.77.11.6749

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

27 in total

1. Requirement of a functional gene 32 product of bacteriophage T4 in UV, repair.

Authors: J R Wu; Y C Yeh
Journal: J Virol Date: 1973-10 Impact factor: 5.103

2. Molecular mechanisms of genetic recombination in bacteriophage. VI. A mutant defective in the joining of DNA molecules.

Authors: J I Tomizawa; N Anraku; Y Iwama
Journal: J Mol Biol Date: 1966-11-14 Impact factor: 5.469

3. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. II. Single-strand breaks and exposure of uncomplemented areas as a prerequisite for recombination.

Authors: A W Kozinski; Z Z Felgenhauer
Journal: J Virol Date: 1967-12 Impact factor: 5.103

4. A DNA-binding protein in meiotic cells of Lilium.

Authors: Y Hotta; H Stern
Journal: Dev Biol Date: 1971-09 Impact factor: 3.582

5. T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA.

Authors: B M Alberts; L Frey
Journal: Nature Date: 1970-09-26 Impact factor: 49.962

6. Meiotic protein in spermatocytes of mammals.

Authors: Y Hotta; H Stern
Journal: Nat New Biol Date: 1971-11-17

7. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments.

Authors: M Ashburner
Journal: Chromosoma Date: 1970 Impact factor: 4.316

8. Immunofluorescent labeling of chromosomes with antisera to histones and histone fractions.

Authors: L S Desai; L Pothier; G E Foley; R A Adams
Journal: Exp Cell Res Date: 1972-02 Impact factor: 3.905

9. Analysis of drosophila mRNA by in situ hybridization: sequences transcribed in normal and heat shocked cultured cells.

Authors: A Spradling; S Penman; M L Pardue
Journal: Cell Date: 1975-04 Impact factor: 41.582

Immunoreactive helix-destabilizing protein localized in transcriptionally active regions of Drosophila polytene chromosomes.

1. Requirement of a functional gene 32 product of bacteriophage T4 in UV, repair.

2. Molecular mechanisms of genetic recombination in bacteriophage. VI. A mutant defective in the joining of DNA molecules.

3. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. II. Single-strand breaks and exposure of uncomplemented areas as a prerequisite for recombination.

4. A DNA-binding protein in meiotic cells of Lilium.

5. T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA.

6. Meiotic protein in spermatocytes of mammals.

7. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments.

8. Immunofluorescent labeling of chromosomes with antisera to histones and histone fractions.

9. Analysis of drosophila mRNA by in situ hybridization: sequences transcribed in normal and heat shocked cultured cells.

10. Localization of RNA from heat-induced polysomes at puff sites in Drosophila melanogaster.

1. Phosphotyrosine-containing lactate dehydrogenase is restricted to the nuclei of PC12 pheochromocytoma cells.

2. Isolation of a mouse DNA fraction which encodes more informational than non informational RNA sequences.

3. A destabilized DNA conformation associated with tightly bound nuclear proteins in active genes of rat myoblast.

4. Identification of a nucleic acid helix-destabilizing protein from rat liver as lactate dehydrogenase-5.

5. Manifold expression of new cellular genes in human lymphoid neoplasia.