Literature DB >> 6256172

Binding of 3H-beta-endorphin to rat brain membranes: characterization of opiate properties and interaction with ACTH.

H Akil, W A Hewlett, J D Barchas, C H Li.   

Abstract

The binding of tritiated beta-endorphin (3H-beta-EP) to brain homogenates is described. This has been difficult to achieve due to the lack of availability of 3H-beta-EP and to technical difficulties associated with high non-specific binding of beta-EP. We now report that 3H-beta-EP binding is saturable, stereospecific, has high affinity and is inhibited by sodium. Its dissociation rate is ten-fold longer than that of naloxone. Its regional distribution exhibits interesting differences from naloxone and enkephalin binding. ACTH1-24 appears to displace it more effectively than it displaces 3H-naloxone. The results are discussed in terms of multiple transmitter systems and the multiple opiate receptor hypothesis.

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Year:  1980        PMID： 6256172     DOI： 10.1016/0014-2999(80)90363-5

Source DB:  PubMed          Journal:  Eur J Pharmacol        ISSN： 0014-2999            Impact factor:   4.432


  9 in total

1.  Opioid peptides as neuroregulators: potential areas for the study of genetic-behavioral mechanisms.

Authors:  J D Barchas; S Sullivan
Journal:  Behav Genet       Date:  1982-02       Impact factor: 2.805

2.  Characterization of solubilized human and rat brain beta-endorphin-receptor complex.

Authors:  D M Helmeste; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

3.  Comparative study of the roles of ACTH and beta-endorphin in regulating conditioned reflex activity in the hedgehog.

Authors:  T N Sollertinskaya
Journal:  Neurosci Behav Physiol       Date:  1997 Nov-Dec

4.  Preparation of [125I-Tyr27,Leu5]beta h-endorphin and its use for crosslinking of opioid binding sites in human striatum and NG108-15 neuroblastoma-glioma cells.

Authors:  D M Helmeste; R G Hammonds; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

5.  beta-Endorphin: characterization of binding sites specific for the human hormone in human glioblastoma SF126 cells.

Authors:  M Westphal; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

6.  beta-Endorphin-induced analgesia is inhibited by synthetic analogs of beta-endorphin.

Authors:  P Nicolas; R G Hammonds; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

7.  A dose-response analysis of the beneficial effects of the ACTH-(4-9) analogue, Org 2766, on behavioural recovery following unilateral labyrinthectomy in guinea-pig.

Authors:  D P Gilchrist; C L Darlington; P F Smith
Journal:  Br J Pharmacol       Date:  1994-01       Impact factor: 8.739

8.  Central delta-opioid receptor interactions and the inhibition of reflex urinary bladder contractions in the rat.

Authors:  A Dray; L Nunan; W Wire
Journal:  Br J Pharmacol       Date:  1985-07       Impact factor: 8.739

Review 9.  Peptide neuroregulators: the opioid system as a model.

Authors:  J D Barchas; C Evans; G R Elliott; P A Berger
Journal:  Yale J Biol Med       Date:  1985 Nov-Dec
  9 in total

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