Literature DB >> 6255407

Selective blotting of restriction DNA fragments on nitrocellulose membranes at low salt concentrations.

Y Nagamine, A Sentenac, P Fromageot.   

Abstract

The pattern of restriction DNA fragments transferred from agarose gel to nitrocellulose membranes (Southern's technique) can be affected by the salt concentration of the transfer solvent.

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Year:  1980        PMID: 6255407      PMCID: PMC324093          DOI: 10.1093/nar/8.11.2453

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  20 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Effect of salts and polyamines on T4 polynucleotide kinase.

Authors:  J R Lillehaug; K Kleppe
Journal:  Biochemistry       Date:  1975-03-25       Impact factor: 3.162

3.  Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors:  J C Alwine; D J Kemp; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

4.  A very large repeating unit of mouse DNA containing the 18S, 28S and 5.8S rRNA genes.

Authors:  S Cory; J M Adams
Journal:  Cell       Date:  1977-08       Impact factor: 41.582

5.  Plasmid-linked ampicillin resistance in haempohilus influenza type b.

Authors:  L P Elwell; J De Graaff; D Seibert; S Falkow
Journal:  Infect Immun       Date:  1975-08       Impact factor: 3.441

6.  Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.

Authors:  G Ketner; T J Kelly
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

7.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

8.  Cloned Bacillus subtilis DNA containing a gene that is activated early during sporulation.

Authors:  J Segall; R Losick
Journal:  Cell       Date:  1977-08       Impact factor: 41.582

9.  Patterns of integration of viral DNA sequences in the genomes of adenovirus type 12-transformed hamster cells.

Authors:  D Sutter; M Westphal; W Doerfler
Journal:  Cell       Date:  1978-07       Impact factor: 41.582

10.  Heterogeneity of the ribosomal genes in mice and men.

Authors:  N Arnheim; E M Southern
Journal:  Cell       Date:  1977-06       Impact factor: 41.582

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  4 in total

1.  Reverse Southern hybridization.

Authors:  H B Gamper; G D Cimino; S T Isaacs; M Ferguson; J E Hearst
Journal:  Nucleic Acids Res       Date:  1986-12-22       Impact factor: 16.971

2.  Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and analysis of the CEN1-ADE1-CDC15 region.

Authors:  H Y Steensma; J C Crowley; D B Kaback
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

3.  Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome.

Authors:  K G Coleman; H Y Steensma; D B Kaback; J R Pringle
Journal:  Mol Cell Biol       Date:  1986-12       Impact factor: 4.272

4.  Efficient and selective initiation by yeast RNA polymerase B in a dinucleotide-primed reaction.

Authors:  B Lescure; V Williamson; A Sentenac
Journal:  Nucleic Acids Res       Date:  1981-01-10       Impact factor: 16.971

  4 in total

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