| Literature DB >> 6255085 |
Abstract
A tissue culture-adapted strain of bovine rotavirus, grown in calf kidney monolayers, has been examined by light and electron microscopy after immunoperoxidase labelling. Some of the characteristic problems associated with pre-embedding methods have been demonstrated. Preparative techniques involving pretreatment of infected cells with a detergent followed by a detergent/fixative combination have enabled virus antigen to be labelled while maintaining satisfactory ultrastructural preservation.Entities:
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Year: 1980 PMID: 6255085 DOI: 10.1099/0022-1317-50-1-195
Source DB: PubMed Journal: J Gen Virol ISSN: 0022-1317 Impact factor: 3.891