Literature DB >> 6254929

Dehydrogenase activity and loss of formazan from tissue sections.

R G Butcher, A L Dawson, S A Knaab, P B Gahan.   

Abstract

Formazans can be lost from tissue sections when the incubation medium is removed at the end of a dehydrogenase reaction. The loss is not uniform and will clearly affect the interpretation of results. This suggests that both the qualitative evaluation of the reaction and the measurement of enzyme activity should be made at the time of incubation.

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Year:  1980        PMID： 6254929     DOI： 10.1007/bf01011932

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  6 in total

1.  Precise cytochemical measurement of neotetrazolium formazan by scanning and integrating microdensitometry.

Authors:  R G Butcher
Journal:  Histochemie       Date:  1972

2.  A gelatin film method for improved histochemical localization of dehydrogenases in plant cells.

Authors:  M Kalina; P B Gahan
Journal:  Stain Technol       Date:  1968-03

3.  Studies on the reduction of tetrazolium salts. II. The measurement of the half reduced and fully reduced formazans of neotetrazolium chloride in tissue sections.

Authors:  R G Butcher; F P Altman
Journal:  Histochemie       Date:  1973-12-31

4.  The use of tetrazolium salts in the histochemical demonstration of succinic dehydrogenase activity in plant tissues.

Authors:  P B Gahan; M Kalina
Journal:  Histochemie       Date:  1968

5.  The measurement in tissue sections of the two formazans derived from nitroblue tetrazolium in dehydrogenase reactions.

Authors:  R G Butcher
Journal:  Histochem J       Date:  1978-11

6.  Oxygen and the production of formazan from neotetrazolium chloride.

Authors:  R G Butcher
Journal:  Histochemistry       Date:  1978-07-12
  6 in total
  11 in total

1.  Microphotometric determination of enzymes in brain sections. IV. Isocitrate dehydrogenases.

Authors:  P Kugler; S Vogel
Journal:  Histochemistry       Date:  1991

2.  Cytophotometric analysis of reaction rates of succinate and lactate dehydrogenase activity in rat liver, heart muscle and tracheal epithelium.

Authors:  C J Van Noorden; I M Vogels
Journal:  Histochem J       Date:  1989 Sep-Oct

3.  Quantitative histochemistry of creatine kinase in rat myocardium and skeletal muscle.

Authors:  W M Frederiks; F Marx; C J Van Noorden
Journal:  Histochem J       Date:  1988-11

4.  Diffusion during dehydrogenase reactions: the effects of intermediate electron acceptors.

Authors:  R G Butcher; A W Evans
Journal:  Histochem J       Date:  1984-08

5.  Quantitative succinic dehydrogenases histochemistry. A comparison of different tetrazolium salts.

Authors:  H J Lippold
Journal:  Histochemistry       Date:  1982

6.  Succinate dehydrogenase activity in cultured human skin fibroblasts and amniotic fluid cells. A methodological study.

Authors:  T L Hansen; H Andersen
Journal:  Histochemistry       Date:  1983

7.  Reaction rate studies of glucose-6-phosphate dehydrogenase activity in sections of rat liver using four tetrazolium salts.

Authors:  R G Butcher; C J Van Noorden
Journal:  Histochem J       Date:  1985-09

8.  Studies on the phenazine methosulphate-tetrazolium salt capture reaction in NAD(P)+-dependent dehydrogenase cytochemistry. I. Localization artefacts caused by the escape of reduced co-enzyme during cytochemical reactions for NAD(P)+-dependent dehydrogenases.

Authors:  A K Raap; G R Van Hoof; P Van Duijn
Journal:  Histochem J       Date:  1983-09

9.  Studies on the phenazine methosulphate-tetrazolium capture reaction in NAD(P)+-dependent dehydrogenase cytochemistry. II. A novel hypothesis for the mode of action of PMS and a study of the properties of reduced PMS.

Authors:  A K Raap; P Van Duijn
Journal:  Histochem J       Date:  1983-09

10.  On the role of oxygen in dehydrogenase reactions using tetrazolium salts.

Authors:  C J Van Noorden
Journal:  Histochem J       Date:  1988-10
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