Literature DB >> 6254855

Construction of an SV40-derived cloning vector.

N Muzyczka.   

Abstract

A new reiterated variant of simian virus 40 (SV40; dl1142) has been constructed. It should be useful for the purpose of cloning foreign pieces of DNA in SV40 virions. Up to 80% of the SV40 genome has been made available for substitution with foreign DNA and the vector contains a number of unique (single-cut) restriction sites which will facilitate cloning. The 5' and 3' regions of both the SV40 early and late messenger RNAs are included in the vector. Prokaryotic DNA has been successfully cloned in the early region of the vector. The transcriptional properties of the recombinant have been studied, and it was found that both the vector and insert DNA are transcribed, mainly as non-adenylated RNA.

Entities:  

Mesh：

Year:  1980        PMID： 6254855     DOI： 10.1016/0378-1119(80)90087-6

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  10 in total

1.  Characterization of am404, an amber mutation in the simian virus 40 T antigen gene.

Authors:  D R Rawlins; P Collis; N Muzyczka
Journal:  J Virol       Date:  1983-07       Impact factor: 5.103

2.  A cis-acting sequence promotes removal of simian virus 40 DNA from the replication pool.

Authors:  H T Wang; S H Larsen; A Roman
Journal:  J Virol       Date:  1985-02       Impact factor: 5.103

3.  In vitro excision of adeno-associated virus DNA from recombinant plasmids: isolation of an enzyme fraction from HeLa cells that cleaves DNA at poly(G) sequences.

Authors:  J Gottlieb; N Muzyczka
Journal:  Mol Cell Biol       Date:  1988-06       Impact factor: 4.272

4.  Genetics of adeno-associated virus: isolation and preliminary characterization of adeno-associated virus type 2 mutants.

Authors:  P L Hermonat; M A Labow; R Wright; K I Berns; N Muzyczka
Journal:  J Virol       Date:  1984-08       Impact factor: 5.103

5.  Genetic analysis of adeno-associated virus: properties of deletion mutants constructed in vitro and evidence for an adeno-associated virus replication function.

Authors:  J D Tratschin; I L Miller; B J Carter
Journal:  J Virol       Date:  1984-09       Impact factor: 5.103

6.  Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector.

Authors:  K Oda; H Kato; I Saito; S Sugano; K Maruyama; M Masuda; K Shiroki; H Shimojo
Journal:  J Virol       Date:  1983-01       Impact factor: 5.103

7.  Cloning of adeno-associated virus into pBR322: rescue of intact virus from the recombinant plasmid in human cells.

Authors:  R J Samulski; K I Berns; M Tan; N Muzyczka
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

8.  Use of adeno-associated virus as a mammalian DNA cloning vector: transduction of neomycin resistance into mammalian tissue culture cells.

Authors:  P L Hermonat; N Muzyczka
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

9.  A human parvovirus, adeno-associated virus, as a eucaryotic vector: transient expression and encapsidation of the procaryotic gene for chloramphenicol acetyltransferase.

Authors:  J D Tratschin; M H West; T Sandbank; B J Carter
Journal:  Mol Cell Biol       Date:  1984-10       Impact factor: 4.272

10.  Adeno-associated virus general transduction vectors: analysis of proviral structures.

Authors:  S K McLaughlin; P Collis; P L Hermonat; N Muzyczka
Journal:  J Virol       Date:  1988-06       Impact factor: 5.103
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.