Literature DB >> 6254852

A cautionary note on the use of certain restriction endonucleases with methylated substrates.

K Backman.   

Abstract

Methylation of adenine and cytosine residues in DNA isolated from common strains of Escherichia coli K-12 can render that DNA resistant to cleavage by certain restriction endonucleases at those sites at which the recognition sequence for such an endonuclease overlaps (but does not include) a sequence recognized by methylases specified by the dam or dcm gene.

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Year:  1980        PMID： 6254852     DOI： 10.1016/0378-1119(80)90097-9

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  26 in total

1.  Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors:  M McClelland; M Nelson
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

2.  Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors:  M Nelson; M McClelland
Journal:  Nucleic Acids Res       Date:  1989       Impact factor: 16.971

Review 3.  The effect of site-specific methylation on restriction-modification enzymes.

Authors:  M Nelson; M McClelland
Journal:  Nucleic Acids Res       Date:  1987       Impact factor: 16.971

4.  The effect of site specific methylation on restriction endonuclease digestion.

Authors:  M McClelland; M Nelson
Journal:  Nucleic Acids Res       Date:  1985       Impact factor: 16.971

5.  Herpes simplex virus amplicon: cleavage of concatemeric DNA is linked to packaging and involves amplification of the terminally reiterated a sequence.

Authors:  L P Deiss; N Frenkel
Journal:  J Virol       Date:  1986-03       Impact factor: 5.103

6.  Alteration of apparent restriction endonuclease recognition specificities by DNA methylases.

Authors:  M Nelson; C Christ; I Schildkraut
Journal:  Nucleic Acids Res       Date:  1984-07-11       Impact factor: 16.971

7.  Cloning and expression of beta-glucosidase genes in Escherichia coli and Saccharomyces cerevisiae using shuttle vector pYES 2.0.

Authors:  M I Rajoka; A Bashir; S R Hussain; M T Ghauri; S Parvez; K A Malik
Journal:  Folia Microbiol (Praha)       Date:  1998       Impact factor: 2.099

8.  Control of Herpes simplex virus thymidine kinase gene expression in Saccharomyces cerevisiae by a yeast promoter sequence.

Authors:  X L Zhu; C Ward; A Weissbach
Journal:  Mol Gen Genet       Date:  1984

9.  Reduced cleavage by sodium hydroxide of methyladenine in DNA sequencing.

Authors:  N Okawa; Y Suyama; A Kaji
Journal:  Nucleic Acids Res       Date:  1985-11-11       Impact factor: 16.971

10.  Cloning, characterization and expression of the gene coding for a cytosine-5-DNA methyltransferase recognizing GpC.

Authors:  M Xu; M P Kladde; J L Van Etten; R T Simpson
Journal:  Nucleic Acids Res       Date:  1998-09-01       Impact factor: 16.971
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