Literature DB >> 6254846

Direct fractionation of genes by preparative electrophoresis of Bacillus subtilis DNA.

K F Bott, C P Moran, M H Edgell, D Bentley, L Charles.   

Abstract

Discontinuous electrophoresis through agarose has been shown to be a satisfactory method for preparation of biologically active restriction fragments from milligram quantities of DNA. The DNA is obtained in sufficient quantity for: (1) direct use in genetic transformation, (2) the production of multiple-dimensional restriction analyses, or (3) use as a high-resolution hybridization probe.

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Year:  1980        PMID: 6254846     DOI: 10.1016/0378-1119(80)90057-8

Source DB:  PubMed          Journal:  Gene        ISSN: 0378-1119            Impact factor:   3.688


  3 in total

1.  Genetic and physical organization of the cloned gyrA and gyrB genes of Bacillus subtilis.

Authors:  M F Lampe; K F Bott
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

2.  Cloning the gyrA gene of Bacillus subtilis.

Authors:  M F Lampe; K F Bott
Journal:  Nucleic Acids Res       Date:  1984-08-10       Impact factor: 16.971

3.  Isolation of an autonomously replicating DNA fragment from the region of defective bacteriophage PBSX of Bacillus subtilis.

Authors:  L M Anderson; H E Ruley; K F Bott
Journal:  J Bacteriol       Date:  1982-06       Impact factor: 3.490

  3 in total

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