Literature DB >> 6254045

DNA-transformed murine teratocarcinoma cells: regulation of expression of simian virus 40 tumor antigen in stem versus differentiated cells.

A Linnenbach, K Huebner, C M Croce.   

Abstract

Thymidine kinase-deficient (TK-; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21)F9 teratocarcinoma stem cells have been transformed with a recombinant plasmid genome consisting of the pBR322 genome linked to a herpes simplex virus type 1 thymidine kinase gene (HSV-1 tk) and a simian virus 40 (SV40) genome. A clonal line of stem cells was obtained that contains only one copy of plasmid DNA, which is integrated into murine chromosomal DNA through a site on the pBRR322 genome. The HSV-1 tk gene, which is adjacent to the SV40 genome, is expressed in stem cells, whereas SV40 gene expression is not detectable. If differentiation of these stem cells is induced, the differentiated cells express SV40 early gene products. Thus, we have constructed a stem cell which contains a set of genes (SV40), the expression of which is regulated differently in stem and differentiated cells. This cell line could be used to determine the mechanism of suppression of expression of these genes in stem cells.

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Year:  1980        PMID： 6254045      PMCID： PMC349951          DOI： 10.1073/pnas.77.8.4875

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Neoplastic differentiation: interaction of simian virus 40 and polyoma virus with murine teratocarcinoma cells in vitro.

Authors:  D E Swartzendruber; J M Lehman
Journal:  J Cell Physiol       Date:  1975-04       Impact factor: 6.384

4.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

5.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

6.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

7.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

8.  The nature of thymidine kinase in the human-mouse hybrid cell.

Authors:  B R Migeon; S W Smith; C L Leddy
Journal:  Biochem Genet       Date:  1969-12       Impact factor: 1.890

9.  A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.

Authors:  R Radloff; W Bauer; J Vinograd
Journal:  Proc Natl Acad Sci U S A       Date:  1967-05       Impact factor: 11.205

10.  Amplification and characterization of a beta-globin gene synthesized in vitro.

Authors:  T Maniatis; S G Kee; A Efstratiadis; F C Kafatos
Journal:  Cell       Date:  1976-06       Impact factor: 41.582
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  24 in total

1.  Adenovirus infection of differentiated F9 cells results in a global shut-off of differentiation-induced gene expression.

Authors:  R J Weigel; J R Nevins
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

2.  The state of cellular differentiation determines the activity of the adenovirus E1A enhancer element: evidence for negative regulation of enhancer function.

Authors:  R S Herbst; M Pelletier; E M Boczko; L E Babiss
Journal:  J Virol       Date:  1990-01       Impact factor: 5.103

3.  High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion.

Authors:  R M Sandri-Goldin; A L Goldin; M Levine; J C Glorioso
Journal:  Mol Cell Biol       Date:  1981-08       Impact factor: 4.272

4.  A cellular enhancer of retrovirus gene expression in embryonal carcinoma cells.

Authors:  M Taketo; M Tanaka
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

5.  The regulation of SV40 early gene expression in embryonal carcinoma stem cells--faithful transcriptional regulation in vitro.

Authors:  N B La Thangue; P W Rigby
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971

6.  NF-kappa B-independent activation of beta-interferon expression in mouse F9 embryonal carcinoma cells.

Authors:  M J Ellis; S Goodbourn
Journal:  Nucleic Acids Res       Date:  1994-10-25       Impact factor: 16.971

7.  Control of H-2 antigen and beta 2-microglobulin gene expression in mouse trophoblast cell clones.

Authors:  K Tanaka; K Ozato; G Jay; J R Parnes; L Ramanathan; J G Seidman; K S Chang; E Appella
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

8.  Lack of retrovirus gene expression in somatic cell hybrids of friend cells and teratocarcinoma cells with a teratocarcinoma phenotype.

Authors:  W Asche; G Colletta; G Warnecke; P Nobis; S Pennie; R M King; W Ostertag
Journal:  Mol Cell Biol       Date:  1984-05       Impact factor: 4.272

9.  Expression of simian virus 40 large T antigen in embryonal carcinoma cell hybrids.

Authors:  A Tunnacliffe; L V Crawford; P Goodfellow
Journal:  Mol Cell Biol       Date:  1984-08       Impact factor: 4.272

10.  Transfer of nonselectable genes into mouse teratocarcinoma cells and transcription of the transferred human beta-globin gene.

Authors:  E F Wagner; B Mintz
Journal:  Mol Cell Biol       Date:  1982-02       Impact factor: 4.272
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