Literature DB >> 6253143

Origin-defective mutants of SV40.

Y Gluzman, R J Frisque, J Sambrook.   

Abstract

We have described a new technique for the isolation and propagation of nonconditionally lethal mutants. We have used this method to generate mutants of SV40 that contain a defective origin of DNA replication. Using these mutants, we have established the following: (1) SV40 DNA replication and early transcription are functionally separate. (2) A functional viral origin of DNA replication is not necessary for the maintenance of transformation. (3) The lack of the origin of SV40 DNA replication does not affect the efficiency of transformation when nonpermissive cells are transfected by DNA using the calcium technique.

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Year:  1980        PMID： 6253143     DOI： 10.1101/sqb.1980.044.01.033

Source DB:  PubMed          Journal:  Cold Spring Harb Symp Quant Biol        ISSN： 0091-7451


  88 in total

1.  Resistance to oncogenic transformation in revertant R1 of human ras-transformed NIH 3T3 cells.

Authors:  N Kuzumaki; Y Ogiso; A Oda; H Fujita; H Suzuki; C Sato; L Müllauer
Journal:  Mol Cell Biol       Date:  1989-05       Impact factor: 4.272

2.  Immortalization of human fibroblasts transformed by origin-defective simian virus 40.

Authors:  D S Neufeld; S Ripley; A Henderson; H L Ozer
Journal:  Mol Cell Biol       Date:  1987-08       Impact factor: 4.272

3.  A specific signature of Merkel cell polyomavirus persistence in human cancer cells.

Authors:  Harald zur Hausen
Journal:  Proc Natl Acad Sci U S A       Date:  2008-10-15       Impact factor: 11.205

4.  Linker insertion mutants of simian virus 40 large T antigen that show trans-dominant interference with wild-type large T antigen map to multiple sites within the T-antigen gene.

Authors:  J Y Zhu; C N Cole
Journal:  J Virol       Date:  1989-11       Impact factor: 5.103

5.  Simian virus 40 origin- and T-antigen-dependent DNA replication with Drosophila factors in vitro.

Authors:  R T Kamakaka; P D Kaufman; B Stillman; P G Mitsis; J T Kadonaga
Journal:  Mol Cell Biol       Date:  1994-08       Impact factor: 4.272

6.  Extended life span of human endometrial stromal cells transfected with cloned origin-defective, temperature-sensitive simian virus 40.

Authors:  C A Rinehart; J S Haskill; J S Morris; T D Butler; D G Kaufman
Journal:  J Virol       Date:  1991-03       Impact factor: 5.103

7.  Transfection with extracellularly UV-damaged DNA induces human and rat cells to express a mutator phenotype towards parvovirus H-1.

Authors:  C Dinsart; J J Cornelis; B Klein; A J van der Eb; J Rommelaere
Journal:  Mol Cell Biol       Date:  1984-02       Impact factor: 4.272

8.  Immortalization of normal human gingival keratinocytes and cytological and cytogenetic characterization of the cells.

Authors:  Chikahiro Kubo; Takeo W Tsutsui; Yukiko Tamura; Shin-Ichi Kumakura; Takeki Tsutsui
Journal:  Odontology       Date:  2009-01-29       Impact factor: 2.634

9.  Essential nucleotides in the polyomavirus origin region.

Authors:  S J Triezenberg; W R Folk
Journal:  J Virol       Date:  1984-08       Impact factor: 5.103

10.  Identification of the replicative intermediates in SV40 DNA replication in vitro.

Authors:  H Ariga
Journal:  Nucleic Acids Res       Date:  1984-08-10       Impact factor: 16.971
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