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Literature DB >> 6252292

Enzyme-linked immunosorbent assay for coronaviruses HCV 229E and MHV 3.

C A Kraaijeveld, M H Madge, M R Macnaughton.

Abstract

The antigenic relationship between human cornonavirus strain 229E (HCV 229E) and mouse hepatitis virus strain 3 (MHV 3) was studied by means of the indirect form of enzyme-linked immunosorbent assay (ELISA). A cross-reaction was found with hyprimmune rabbit sera between HCV 229E and MHV 3 which may be due to the adherence of bovine serum componeants from tissue culture media, which were present on virus particles even after extensive purification. No cross-reaction was observed with immune sera absorbed with bovine serum, or with HCV 229E grown in tissue culture without serum. This indirect ELISA with HCV 229E may prove to be useful for studies with human sera.

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Year: 1980 PMID： 6252292 DOI： 10.1099/0022-1317-49-1-83

Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891

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Cited

18 in total

1. Effect of specific humoral immunity and some non-specific factors on resistance of volunteers to respiratory coronavirus infection.

Authors: K A Callow
Journal: J Hyg (Lond) Date: 1985-08

2. Porcine epidemic diarrhea virus (CV 777) and feline infectious peritonitis virus (FIPV) are antigenically related.

Authors: Y L Zhou; J Ederveen; H Egberink; M Pensaert; M C Horzinek
Journal: Arch Virol Date: 1988 Impact factor: 2.574

3. Antibody to virus components in volunteers experimentally infected with human coronavirus 229E group viruses.

Authors: M R Macnaughton; H J Hasony; M H Madge; S E Reed
Journal: Infect Immun Date: 1981-03 Impact factor: 3.441

4. A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus.

Authors: J L Leibowitz; L S Fung; G A Levy
Journal: J Virol Methods Date: 1983-05 Impact factor: 2.014

5. Role of recent vaccination in production of false-positive coronavirus antibody titers in cats.

Authors: J E Barlough; R H Jacobson; C E Pepper; F W Scott
Journal: J Clin Microbiol Date: 1984-03 Impact factor: 5.948

6. Detection by radioimmunoassay and enzyme-linked immunosorbent assay of coronavirus antibodies in bovine serum and lacteal secretions.

Authors: L Rodak; L A Babiuk; S D Acres
Journal: J Clin Microbiol Date: 1982-07 Impact factor: 5.948

Enzyme-linked immunosorbent assay for coronaviruses HCV 229E and MHV 3.

1. Effect of specific humoral immunity and some non-specific factors on resistance of volunteers to respiratory coronavirus infection.

2. Porcine epidemic diarrhea virus (CV 777) and feline infectious peritonitis virus (FIPV) are antigenically related.

3. Antibody to virus components in volunteers experimentally infected with human coronavirus 229E group viruses.

4. A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus.

5. Role of recent vaccination in production of false-positive coronavirus antibody titers in cats.

6. Detection by radioimmunoassay and enzyme-linked immunosorbent assay of coronavirus antibodies in bovine serum and lacteal secretions.

7. Development and application of an enzyme immunoassay for coronavirus OC43 antibody in acute respiratory illness.

8. Some recent work at the Common Cold Unit, Salisbury.

9. Pleural effusion disease in rabbits. Properties of the aetiological agent.

10. Epidemiology of coronavirus respiratory infections.