| Literature DB >> 6251158 |
A H Bingham, C J Bruton, T Atkinson.
Abstract
A restriction endonuclease cleavage map of the tetracycline resistance plasmid pAB124, originally isolated from Bacillus stearothermophilus, was constructed using ten enzymes. Tetracycline resistance was associated with a 1 x 95 megadalton (Md) region of pAB124 lying between two EcoRI sites, and this region was circularized to produce a viable tetracycline resistance plasmid (pAB224), with two EcoRI fragments of pAB124 deleted amounting to 0 x 95 Md. A second plasmid (pAB524) with one EcoRI fragment (0 x 6 Md) of pAB124 deleted was also constructed. Restriction endonuclease cleavage maps of pAB224 and pAB524 were constructed.Entities:
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Year: 1980 PMID： 6251158 DOI： 10.1099/00221287-119-1-109
Source DB: PubMed Journal: J Gen Microbiol ISSN： 0022-1287