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Literature DB >> 6251090

Purification and structural properties of gelsolin, a Ca2+-activated regulatory protein of macrophages.

Abstract

We describe the purification procedure and some of the physiochemical properties of gelsolin, a major Ca2+-dependent regulatory protein of actin gel-sol transformation in rabbit lung macrophages. Gelsolin accounts for the majority of Ca2+ control of actin gelation in macrophage extracts. It is a single polypeptide chain with an average molecular weight of 91,000 a Stokes radius of 44 A, a sedimentation coefficient (s20(0),w) of 4.9 S, an isoelectric point of 6.1, and a frictional ratio of 1.43. Gelsolin binds 2 mol of Ca2+ with high affinity (Ka 1.09 X 10(6) M-1) in the presence of 0.1 M KCl and 2 mM MgCl2.

Entities: Chemical Gene Species

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Year: 1980 PMID： 6251090

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

47 in total

1. Accelerators, Brakes, and Gears of Actin Dynamics in Dendritic Spines.

Authors: Crystal G Pontrello; Iryna M Ethell
Journal: Open Neurosci J Date: 2009-01-01

2. Role of gelsolin in actin depolymerization of adherent human neutrophils.

Authors: J S Wang; J P Coburn; A I Tauber; K S Zaner
Journal: Mol Biol Cell Date: 1997-01 Impact factor: 4.138

3. Isolation and characterization of gelsolin from cultured BHK cells.

Authors: A J Edgar
Journal: J Muscle Res Cell Motil Date: 1989-12 Impact factor: 2.698

4. Role of group-specific component (vitamin D binding protein) in clearance of actin from the circulation in the rabbit.

Authors: P J Goldschmidt-Clermont; H Van Baelen; R Bouillon; T E Shook; M H Williams; A E Nel; R M Galbraith
Journal: J Clin Invest Date: 1988-05 Impact factor: 14.808

Purification and structural properties of gelsolin, a Ca2+-activated regulatory protein of macrophages.

1. Accelerators, Brakes, and Gears of Actin Dynamics in Dendritic Spines.

2. Role of gelsolin in actin depolymerization of adherent human neutrophils.

3. Isolation and characterization of gelsolin from cultured BHK cells.

4. Role of group-specific component (vitamin D binding protein) in clearance of actin from the circulation in the rabbit.

5. Inhibition of apoptosis by the actin-regulatory protein gelsolin.

6. A novel pathway for Ca2+ signalling in neutrophils by immune complexes.

Review 7. The lens actin filament cytoskeleton: Diverse structures for complex functions.

8. The Ca(2+)-induced conformational change of gelsolin is located in the carboxyl-terminal half of the molecule.

9. Definition of the EGTA-independent interface involved in the serum gelsolin-actin complex.

10. Distribution of gelsolin in the retina of the developing rabbit.