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Literature DB >> 6250473

Improved method for purification of enterotoxin from Clostridium perfringens type A.

Abstract

The purification procedure of Clostridium perfringens type A enterotoxin has been improved. The cell sonic extract was precipitated twice with ammonium sulfate, first 40% saturated to concentrate the enterotoxin and then 15% saturated. The two precipitations were followed by gel filtration on Sephadex G-100. The enterotoxin appeared to be homogeneous on 7% polyacrylamide gel electrophoresis after this three-step purification procedure, with a recovery of 56% and a 12.3-fold purification. The solubility properties at different pH values, temperatures, and ammonium sulfate concentrations are also given as basis for the purification procedure.

Entities: Chemical Species

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Year: 1980 PMID： 6250473 PMCID： PMC291492 DOI： 10.1128/aem.39.6.1120-1122.1980

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

6 in total

1. A rapid micromethod for estimation of non-volatile organic matter.

Authors: M J JOHNSON
Journal: J Biol Chem Date: 1949-12 Impact factor: 5.157

2. Purification and biochemical properties of Clostridium perfringens type A enterotoxin.

Authors: R L Stark; C L Duncan
Journal: Infect Immun Date: 1972-11 Impact factor: 3.441

3. Purification and characteristics of the enterotoxin of Clostridium perfringens type A.

Authors: A H Hauschild; R Hilsheimer
Journal: Can J Microbiol Date: 1971-11 Impact factor: 2.419

4. Simplified method for purification of Clostridium perfringens type A enterotoxin.

Authors: G Sakaguchi; T Uemura; H P Riemann
Journal: Appl Microbiol Date: 1973-11

5. Chemical modification and characterization of enterotoxin from clostridium perfringens type A.

Authors: P E Granum; R Skjelkvåle
Journal: Acta Pathol Microbiol Scand B Date: 1977-02

6. Improved medium for sporulation of Clostridium perfringens.

Authors: C L Duncan; D H Strong
Journal: Appl Microbiol Date: 1968-01

6 in total

15 in total

1. Sporulation and enterotoxin production by Clostridium perfringens type A at 37 and 43 degrees C.

Authors: J S Garcia-Alvarado; R G Labbé; M A Rodriguez
Journal: Appl Environ Microbiol Date: 1992-04 Impact factor: 4.792

2. Morphological alterations and changes in cellular cations induced by Clostridium perfringens type A enterotoxin in tissue culture cells.

Authors: N Sugimoto; K Ozutsumi; M Matsuda
Journal: Eur J Epidemiol Date: 1985-12 Impact factor: 8.082

3. Proteolysis of Clostridium perfringens type A enterotoxin during purification.

Authors: K B Park; R G Labbé
Journal: Infect Immun Date: 1990-06 Impact factor: 3.441

4. Purification by high performance liquid chromatography of Clostridium perfringens type A enterotoxin prepared from high toxin producers selected by a toxin-antitoxin halo.

Authors: N Sugimoto; K Ozutsumi; M Matsuda
Journal: Eur J Epidemiol Date: 1985-06 Impact factor: 8.082

5. Characterization of a parasporal inclusion body from sporulating, enterotoxin-positive Clostridium perfringens type A.

Authors: A Löffler; R Labbé
Journal: J Bacteriol Date: 1986-02 Impact factor: 3.490

10. Purification of two Clostridium perfringens enterotoxin-like proteins and their effects on membrane permeability in primary cultures of adult rat hepatocytes.

Authors: B R Dasgupta; M W Pariza
Journal: Infect Immun Date: 1982-11 Impact factor: 3.441