Literature DB >> 624835

Measurement of cellular DNA mass by flow microfluorometry with use of a biological internal standard.

E Tannenbaum, M Cassidy, O Alabaster, C Herman.   

Abstract

Use of a biological standard (chicken erythrocytes) mixed with experimental cell populations allows control of all variables in flow microfluorometric determination of DNA content. These variables include both staining and instrument procedures. In addition, the use of a biological standard allows determination of cellular DNA mass in unperturbed cell populations. DNA mass measured by FMF technique correlates closely with values reported in the literature that used biochemical techniques.

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Year:  1978        PMID: 624835     DOI: 10.1177/26.2.624835

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN: 0022-1554            Impact factor:   2.479


  6 in total

1.  Flowcytometric and cytogenetic analysis of human cultured cell lines derived from high- and low-grade astrocytomas.

Authors:  N Shitara; P E McKeever; J Whang-Peng; T Knutsen; B H Smith; P L Kornblith
Journal:  Acta Neuropathol       Date:  1983       Impact factor: 17.088

2.  Genome evolution in pocket gophers (genus Thomomys). II. Variation in cellular DNA content.

Authors:  S W Sherwood; J L Patton
Journal:  Chromosoma       Date:  1982       Impact factor: 4.316

3.  Long-duration intracavitary infusion of methotrexate with systemic leucovorin protection in patients with malignant effusions.

Authors:  S B Howell; B B Chu; W E Wung; B M Metha; J Mendelsohn
Journal:  J Clin Invest       Date:  1981-04       Impact factor: 14.808

4.  Flow cytometric correlation between BrdU/Hoechst quench effect and base pair composition in mammalian cell nuclei.

Authors:  M Kubbies; R Friedl
Journal:  Histochemistry       Date:  1985

5.  The in vitro effect of electromagnetically generated shock waves (Lithostar) on the Dunning R3327 PAT-2 rat prostatic cancer cell-line. A potentiating effect on the in vitro cytotoxicity of vinblastin.

Authors:  G O Oosterhof; G A Smits; J E de Ruyter; R J van Moorselaar; J A Schalken; F M Debruyne
Journal:  Urol Res       Date:  1989

6.  Establishment and characterization of bovine mammary epithelial cell lines.

Authors:  C A Gibson; J R Vega; C R Baumrucker; C S Oakley; C W Welsch
Journal:  In Vitro Cell Dev Biol       Date:  1991-07
  6 in total

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