Literature DB >> 6247437

Indirect enzyme-linked immunosorbent assay (ELISA) for the detection of Coxsackievirus group B antibodies.

M G Katze, R L Crowell.   

Abstract

An indirect, solid phase, microplate enzyme-linked immunosorbent assay (ELISA) was found to be highly sensitive and reliable for detecting antibodies to the group B Coxsackieviruses and other picornaviruses. Conditions for obtaining maximum sensitivity and reproducibility of the indirect ELISA are described. Antibody titres were comparable to those obtained by the virus neutralization test and over 50 times higher than those obtained by the complement-fixation test. Purified virions used in the indirect ELISA reacted with low levels of cross-reacting heterotypic antibodies elicited by each of the six group B Coxsackieviruses, although homotypic reactions resulted in highest titres.

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Year:  1980        PMID： 6247437     DOI： 10.1099/0022-1317-48-1-225

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  7 in total

1.  Identification of group-common linear epitopes in structural and nonstructural proteins of enteroviruses by using synthetic peptides.

Authors:  J Cello; A Samuelson; P Stålhandske; B Svennerholm; S Jeansson; M Forsgren
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

2.  Binding of neutralizing monoclonal antibodies to empty capsids of poliovirus can be blocked by monospecific antisera to structural polypeptides VP1 and VP2.

Authors:  H Uhlig; F Haardt; R Dernick
Journal:  Arch Virol       Date:  1985       Impact factor: 2.574

3.  Cross-reactions of immunoglobulin M and G antibodies with enterovirus-specific viral structural proteins.

Authors:  F Reigel; F Burkhardt; U Schilt
Journal:  J Hyg (Lond)       Date:  1985-10

4.  Altered receptor specificity of coxsackievirus B3 after growth in rhabdomyosarcoma cells.

Authors:  K J Reagan; B Goldberg; R L Crowell
Journal:  J Virol       Date:  1984-03       Impact factor: 5.103

5.  Tests for coxsackie B virus-specific IgM.

Authors:  J R Pattison
Journal:  J Hyg (Lond)       Date:  1983-06

6.  Coxsackievirus B1-based antibody-capture enzyme-linked immunosorbent assay for detection of immunoglobulin G (IgG), IgM, and IgA with broad specificity for enteroviruses.

Authors:  C M Swanink; L Veenstra; Y A Poort; J A Kaan; J M Galama
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

7.  Antibody capture radioimmunoassay (MACRIA) for coxsackievirus B4 and B5-specific IgM.

Authors:  P Morgan-Capner; C McSorley
Journal:  J Hyg (Lond)       Date:  1983-06
  7 in total

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