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Literature DB >> 6246888

Subcellular localization of chicken kidney aryl acylamidase activity.

Abstract

The intracellular localization of aryl acylamidase (aryl-acylamide amidohydrolase, EC 3.5.1.13) in chicken kidney was investigated. By separation on density gradients of the silica sol Ludox AM, the enzyme was localized in the mitochondrial fraction. This mitochondrial fraction was shown to be substantially free of lysosomal contamination. Subfractionation of the purified mitochondria indicates that the enzyme is located on the outer membrane, can be solubilized, and may be a suitable marker enzyme for kidney mitochondria.

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Year: 1980 PMID： 6246888 PMCID： PMC1161603 DOI： 10.1042/bj1860507

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

22 in total

9. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.

Authors: G L Sottocasa; B Kuylenstierna; L Ernster; A Bergstrand
Journal: J Cell Biol Date: 1967-02 Impact factor: 10.539

10. Lysosomes in skeletal muscle tissue. Zonal centrifugation evidence for multiple cellular sources.

Authors: P G Canonico; J W Bird
Journal: J Cell Biol Date: 1970-05 Impact factor: 10.539

1 in total

1. Subcellular localization of rice leaf aryl acylamidase activity.

Authors: J J Gaynor; C C Still
Journal: Plant Physiol Date: 1983-05 Impact factor: 8.340

1 in total

Subcellular localization of chicken kidney aryl acylamidase activity.

1. Aryl acylamidase activity in larvae of the webbing clothes moth, Tineola bisselliella.

2. Factors affecting the separation of photosynthetically competent chloroplasts in gradients of silica sols.

3. The separation of cells and subcellular particles by colloidal silica density gradient centrifugation.

4. The purification of lysosomes from HeLa cells by centrifugation in colloidal silica density gradients.

5. Gradient centrifugation in colloidal silica-polysaccharide media.

6. Arylamidases of rat liver and kidney.

7. Enzyme detoxication of 3',4'-dichloropropionanilide in rice and barnyard grass, a factor in herbicide selectivity.

8. Herbicide transformation. II. Studies with an acylamidase of Fusarium solani.

9. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.

10. Lysosomes in skeletal muscle tissue. Zonal centrifugation evidence for multiple cellular sources.

1. Subcellular localization of rice leaf aryl acylamidase activity.