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Literature DB >> 6246405

Identification of a positive regulatory protein in Escherichia coli: the product of the cysB gene.

Abstract

From the specialized transducing bacteriophage lambdacysB, recombinant phages lambdacysB242 and lambdacysB257 have been obtained, each of which carries an amber mutation in the cysB cistron. A comparison of polyacrylamide gel electrophoretic profiles of labelled extracts from uv-irradiated bacteria that had been infected with lambdacysB+ or with lambdacysB-amber phages, led to the identification of a 39,000-dalton polypeptide as the product of the cysB gene. The native protein was purified to near radiochemical purity and was found to be an oligomer with an isoelectric point close to pH 7.

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Year: 1980 PMID： 6246405 DOI： 10.1007/bf00271494

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

14 in total

1. The nature of the product of the cys B gene of Escherichia coli.

Authors: M Tully; M D Yudkin
Journal: Mol Gen Genet Date: 1975

2. Mutations in Escherichia coli that relieve catabolite repression of tryptophanase synthesis. Mutations distant from the tryptophanase gene.

Authors: M D Yudkin
Journal: J Gen Microbiol Date: 1976-01

Identification of a positive regulatory protein in Escherichia coli: the product of the cysB gene.

1. The nature of the product of the cys B gene of Escherichia coli.

2. Mutations in Escherichia coli that relieve catabolite repression of tryptophanase synthesis. Mutations distant from the tryptophanase gene.

3. Analysis of merodiploids of the cysB region in Salmonella typhimurium.

4. Reversible denaturation of enzymes by sodium dodecyl sulfate.

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

6. Characterization of lambdatrp-transducing bacteriophages made in vitro.

7. The construction in vitro of transducing derivatives of phage lambda.

8. Polarity suppressors increase expression of the wild-type tryptophan operon of Escherichia coli.

Review 9. S-amino acid metabolism and its regulation in Escherichia coli and Salmonella typhimurium.

10. Fine-structure mapping and complementation analysis of the Escherichia coli cysB gene.

1. Isolation and nucleotide sequence of the hmp gene that encodes a haemoglobin-like protein in Escherichia coli K-12.

2. Positive regulation of glutamate biosynthesis in Bacillus subtilis.

3. Cloning and physical mapping of the cysB region of Salmonella typhimurium.

4. Identification of the Salmonella typhimurium cysB gene product by two-dimensional protein electrophoresis.

5. Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx.

6. Proteins induced by sulfate limitation in Escherichia coli, Pseudomonas putida, or Staphylococcus aureus.