Literature DB >> 6245871

A new method for the purification of DNA-binding proteins with sequence specificity.

H Weideli, W J Gehring.   

Abstract

We describe a technique for a rapid and efficient isolation and purification of proteins binding to defined DNA sequences. Cloned double-stranded DNA was covalently coupled to m-aminobenzyloximethylcellulose in order to purify proteins which recognize and bind to specific sequences on the DNA. The purification of two DNA-binding proteins from Drosophila melanogaster is demonstrated using the respective cloned DNA sequences.

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Year:  1980        PMID： 6245871     DOI： 10.1111/j.1432-1033.1980.tb04392.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  3 in total

1.  Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene.

Authors:  W K Miskimins; M P Roberts; A McClelland; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

2.  Characterization of Drosophila DNA-binding protein DB-2: demonstration of its sequence-specific interaction with DNA.

Authors:  H Weideli; C Brack; W J Gehring
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

3.  Preferential binding of estrogen-receptor complex to a region containing the estrogen-dependent hypomethylation site preceding the chicken vitellogenin II gene.

Authors:  J P Jost; M Seldran; M Geiser
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205
  3 in total

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