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Literature DB >> 6245639

A simple and fast method for the isolation of basolateral plasma membranes from rat small-intestinal epithelial cells.

V Scalera, C Storelli, C Storelli-Joss, W Haase, H Murer.

Abstract

A method was developed for the analytical and preparative isolation of basolateral plasma membranes from rat small intestine. They were separated on a self-orientating Percoll (modified colloidal silica) gradient starting with a heavy microsomal-membrane fraction and involving centrifugation at 48,000 g for 1 h. (Na+ + K+)-stimulated ATPase activity, used as a marker enzyme for the basolateral plasma membrane, is enriched 20-fold compared with that found in the homogenate of isolated intestinal epithelial cells.

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Year: 1980 PMID： 6245639 PMCID： PMC1161517 DOI： 10.1042/bj1860177

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

11 in total

1. Basolateral plasma membranes of intestinal epithelial cells. Identification by lactoperoxidase-catalysed iodination and isolation after density perturbation with digitonin.

Authors: B A Lewis; A Elkin; R H Michell; R Coleman
Journal: Biochem J Date: 1975-10 Impact factor: 3.857

2. The surface membrane of the small intestinal epithelial cell. I. Localization of adenyl cyclase.

Authors: H Murer; E Ammann; J Biber; U Hopfer
Journal: Biochim Biophys Acta Date: 1976-05-21

3. Analytical isolation of plasma membranes of intestinal epithelial cells: identification of Na, K-ATPase rich membranes and the distribution of enzyme activities.

Authors: A K Mircheff; E M Wright
Journal: J Membr Biol Date: 1976-09-17 Impact factor: 1.843

4. Transport of p-aminohippuric acid by plasma membrane vesicles isolated from rat kidney cortex.

Authors: W Berner; R Kinne
Journal: Pflugers Arch Date: 1976-02-24 Impact factor: 3.657

5. Preparation and characterization of the lateral and basal plasma membranes of the rat intestinal epithelial cell.

Authors: A P Douglas; R Kerley; K J Isselbacher
Journal: Biochem J Date: 1972-08 Impact factor: 3.857

6. Glucose transport in isolated brush-border and lateral-basal plasma-membrane vesicles from intestinal epithelial cells.

Authors: H Murer; U Hopfer; E Kinne-Saffran; R Kinne
Journal: Biochim Biophys Acta Date: 1974-04-29

7. Differential isolation of microvillous and basolateral plasma membranes from intestinal mucosa: mutually exclusive distribution of digestive enzymes and ouabain-sensitive ATPase.

Authors: M Fujita; H Ota; K Kawai; H Matsui; M Nakao
Journal: Biochim Biophys Acta Date: 1972-08-09

8. Distribution of (Na+-K+)-stimulated ATPase activity in rat intestinal mucosa.

Authors: J P Quigley; G S Gotterer
Journal: Biochim Biophys Acta Date: 1969-04

9. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.

Authors: G L Sottocasa; B Kuylenstierna; L Ernster; A Bergstrand
Journal: J Cell Biol Date: 1967-02 Impact factor: 10.539

10. Further biochemical and morphological studies of granule fractions from rabbit heterophil leukocytes.

Authors: M Baggiolini; J G Hirsch; C De Duve
Journal: J Cell Biol Date: 1970-06 Impact factor: 10.539

44 in total

1. Effect of chronic alcohol feeding on physiological and molecular parameters of renal thiamin transport.

Authors: Veedamali S Subramanian; Sandeep B Subramanya; Hidekazu Tsukamoto; Hamid M Said
Journal: Am J Physiol Renal Physiol Date: 2010-04-28

2. Characterization of BADS-binding proteins in epithelial plasma membranes.

Authors: S F Pearce; J A Zadunaisky
Journal: J Membr Biol Date: 1991-09 Impact factor: 1.843

3. Movement of biotin across the rat intestinal basolateral membrane. Studies with membrane vesicles.

Authors: H M Said
Journal: Biochem J Date: 1991-11-01 Impact factor: 3.857

4. Sodium-bicarbonate cotransport occurs in rat kidney cortical membranes but not in rat small intestinal basolateral membranes.

Authors: B Hagenbuch; G Stange; H Murer
Journal: Biochem J Date: 1987-09-01 Impact factor: 3.857

5. Chronic alcohol feeding inhibits physiological and molecular parameters of intestinal and renal riboflavin transport.

Authors: Veedamali S Subramanian; Sandeep B Subramanya; Abhisek Ghosal; Hamid M Said
Journal: Am J Physiol Cell Physiol Date: 2013-06-26 Impact factor: 4.249

6. Chronic alcohol consumption and intestinal thiamin absorption: effects on physiological and molecular parameters of the uptake process.

Authors: Sandeep B Subramanya; Veedamali S Subramanian; Hamid M Said
Journal: Am J Physiol Gastrointest Liver Physiol Date: 2010-05-06 Impact factor: 4.052

7. Chronic alcohol exposure negatively impacts the physiological and molecular parameters of the renal biotin reabsorption process.

Authors: Veedamali S Subramanian; Sandeep B Subramanya; Hamid M Said
Journal: Am J Physiol Renal Physiol Date: 2011-01-05

8. Sodium gradient- and sodium plus potassium gradient-dependent L-glutamate uptake in renal basolateral membrane vesicles.

Authors: B Sacktor; I L Rosenbloom; C T Liang; L Cheng
Journal: J Membr Biol Date: 1981-05-15 Impact factor: 1.843

9. A carrier-mediated transport for folate in basolateral membrane vesicles of rat small intestine.

Authors: H M Said; R Redha
Journal: Biochem J Date: 1987-10-01 Impact factor: 3.857

10. Carbachol- and elevated Ca(2+)-induced translocation of functionally active protein kinase C to the brush border of rabbit ileal Na+ absorbing cells.

Authors: M E Cohen; J Wesolek; J McCullen; K Rys-Sikora; S Pandol; R P Rood; G W Sharp; M Donowitz
Journal: J Clin Invest Date: 1991-09 Impact factor: 14.808