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Literature DB >> 6245059

Assembly of the mitochondrial membrane system: isolation of mitochondrial transfer ribonucleic acid mutants and characterization of transfer ribonucleic acid genes of Saccharomyces cerevisiae.

R E Berlani, C Pentella, G Macino, A Tzagoloff.

Abstract

A method is described for isolating cytoplasmic mutants of Saccharomyces cerevisiae with lesions in mitochondrial transfer ribonucleic acids (tRNA's). The mutants were selected for slow growth on glycerol and for restoration of wild-type growth by cytoplasmic "petite" testers that contain regions of mitochondrial deoxyribonucleic acid (DNA) with tRNA genes. The aminoacylated mitochondrial tRNA's of several presumptive tRNA mutants were analyzed by reverse-phase chromatography on RPC-5. Two mutant strains, G76-26 and G76-35, were determined to carry mutations in the cysteine and histidine tRNA genes, respectively. The cysteine tRNA mutant was used to isolate cytoplasmic petite mutants whose retained segments of mitochondrial DNA contain the cysteine tRNA gene. The segment of one such mutant (DS504) was sequenced and shown to have the cysteine, histidine, and threonine tRNA genes. The structures of the three mitochondrial tRNA's were deduced from the DNA sequence.

Entities: Chemical Gene Species

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Year: 1980 PMID： 6245059 PMCID： PMC293786 DOI： 10.1128/jb.141.3.1086-1097.1980

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

37 in total

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Journal: Nucleic Acids Res Date: 1975-10 Impact factor: 16.971

2. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.

Authors: K Murray; N E Murray
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. Assembly of the mitochondrial membrane system: isolation of nuclear and cytoplasmic mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions.

Authors: A Tzagoloff; A Akai; R B Needleman
Journal: J Bacteriol Date: 1975-06 Impact factor: 3.490

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Authors: P R Avner; D E Griffiths
Journal: Eur J Biochem Date: 1973-01-15

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Authors: J Casey; M Cohen; M Rabinowitz; H Fukuhara; G S Getz
Journal: J Mol Biol Date: 1972-02-14 Impact factor: 5.469

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Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1968-02 Impact factor: 3.162

7. Assembly of the mitochondrial membrane system. Cytoplasmic mutants of Saccharomyces cerevisiae with lesions in enzymes of the respiratory chain and in the mitochondrial ATPase.

Authors: A Tzagoloff; A Akai; R B Needleman; G Zulch
Journal: J Biol Chem Date: 1975-10-25 Impact factor: 5.157

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Authors: G Faye; C Kujawa; H Fukuhara
Journal: J Mol Biol Date: 1974-09-05 Impact factor: 5.469

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Authors: C E Singer; G R Smith
Journal: J Biol Chem Date: 1972-05-25 Impact factor: 5.157

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Authors: J Deutsch; B Dujon; P Netter; E Petrochilo; P P Slonimski; M Bolotin-Fukuhara; D Coen
Journal: Genetics Date: 1974-02 Impact factor: 4.562

29 in total

1. Compilation of tRNA sequences and sequences of tRNA genes.

Authors: M Sprinzl; N Dank; S Nock; A Schön
Journal: Nucleic Acids Res Date: 1991-04-25 Impact factor: 16.971

2. A point mutation in a mitochondrial tRNA gene abolishes its 3' end processing.

Authors: E Zennaro; S Francisci; A Ragnini; L Frontali; M Bolotin-Fukuhara
Journal: Nucleic Acids Res Date: 1989-07-25 Impact factor: 16.971

3. Mutational studies of the major tRNA region of the S. cerevisiae mitochondrial genome.

Authors: N Elelj-Fridhi; C Pallier; R Zelikson; M Guetari; M Bolotin-Fukuhara
Journal: Curr Genet Date: 1991-04 Impact factor: 3.886

4. Nuclear and mitochondrial revertants of a yeast mitochondrial tRNA mutant.

Authors: Y W Kang; D L Miller
Journal: Mol Gen Genet Date: 1988-08

5. Nucleotide sequences of three tRNA genes encoded in Tetrahymena mitochondrial DNA.

Authors: Y Suyama
Journal: Nucleic Acids Res Date: 1985-05-10 Impact factor: 16.971

6. Identification of a new promoter within the tRNA gene cluster of the mitochondrial DNA of Saccharomyces cerevisiae.

Authors: J S Backer; G S Getz
Journal: Nucleic Acids Res Date: 1987-11-25 Impact factor: 16.971

7. Nucleotide sequence of the structural genes for the mitochondrial cys, lys, gln and leu-tRNAs from the yeast Kluyveromyces lactis K8.

Authors: C M Hardy; G D Clark-Walker
Journal: Nucleic Acids Res Date: 1989-02-25 Impact factor: 16.971