Enzyme-linked immunosorbent assay for detection of poliovirus antigen and antibody.

A sensitive method is described to detect poliovirus antigen in diluted samples of semi-processed and processed Salk vaccine material using a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). The introduction of a simple conjugate-blocking step permits confirmation of antigen specificity and allows evaluation of neutralising antibody concentration in the sera employed as soon as four hours after sample addition.