Literature DB >> 6244997

Direct enzyme linked immunosorbent assay (ELISA) for quantification of poliomyelitis virus D-antigen.

M Souvras, B Montagnon, B Fanget, A L van Wezel, A G Hazendonk.   

Abstract

The proposed micro-ELISA assay by means of the double antibody method involves three steps: adsorption of type specific antiserum on micro-wells; simultaneous incubation of antigen and Horse Radish Peroxydase (HRPO) conjugated antiserum; substrate incubation followed by photometric measurement of absorbance at 403 nm. Preliminary results seem generally in good agreement with those obtained by other tests such as gel diffusion and indirect ELISA.

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Year:  1980        PMID: 6244997

Source DB:  PubMed          Journal:  Dev Biol Stand        ISSN: 0301-5149


  2 in total

1.  Evaluation of a poliovirus-binding inhibition assay as an alternative to the virus neutralization test.

Authors:  M M Herremans; J H Reimerink; A Ras; H G Van Der Avoort; T G Kimman; A M Van Loon; M A Conyn-Van Spaendonck; M P Koopmans
Journal:  Clin Diagn Lab Immunol       Date:  1997-11

2.  Detection of poliovirus antigen by enzyme immunoassay.

Authors:  P Payment; M Trudel
Journal:  J Clin Microbiol       Date:  1987-09       Impact factor: 5.948

  2 in total

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