| Literature DB >> 6244997 |
M Souvras, B Montagnon, B Fanget, A L van Wezel, A G Hazendonk.
Abstract
The proposed micro-ELISA assay by means of the double antibody method involves three steps: adsorption of type specific antiserum on micro-wells; simultaneous incubation of antigen and Horse Radish Peroxydase (HRPO) conjugated antiserum; substrate incubation followed by photometric measurement of absorbance at 403 nm. Preliminary results seem generally in good agreement with those obtained by other tests such as gel diffusion and indirect ELISA.Entities:
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Year: 1980 PMID: 6244997
Source DB: PubMed Journal: Dev Biol Stand ISSN: 0301-5149