Literature DB >> 6244716

A radioimmunoassay for cyclic AMP (cAMP) obtained by acetylation of both unlabeled and labeled (3H-cAMP) ligand, or of unlabeled ligand only.

T Skomedal, B Grynne, J B Osnes, A E Sjetnan, I Oye.   

Abstract

A sensitive radioimmunoassay (limit of detection 7 +/- 1 fmol per tube) for cyclic AMP (cAMP) based on acetylation of both 3H-cAMP and unlabeled ligand was developed. Rabbit anti-cAMP antibodies had an apparent Ka for the acetylated ligand of 2 x 10(10) l/mol. When the unlabeled ligand only was acetylated an increased sensitivity was obtained without loss of specificity.

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Year:  1980        PMID： 6244716     DOI： 10.1111/j.1600-0773.1980.tb02443.x

Source DB:  PubMed          Journal:  Acta Pharmacol Toxicol (Copenh)        ISSN： 0001-6683


  11 in total

1.  Effects of insulin on human beta-adrenoceptors.

Authors:  G Sager
Journal:  Br J Clin Pharmacol       Date:  1990       Impact factor: 4.335

2.  Atypical characteristics of the beta-adrenoceptor mediating cyclic AMP generation and lipolysis in the rat adipocyte.

Authors:  D Bojanic; J D Jansen; S R Nahorski; J Zaagsma
Journal:  Br J Pharmacol       Date:  1985-01       Impact factor: 8.739

3.  Differential regulation of β2 -adrenoceptor-mediated inotropic and lusitropic response by PDE3 and PDE4 in failing and non-failing rat cardiac ventricle.

Authors:  Faraz Afzal; Jan Magnus Aronsen; Lise Román Moltzau; Ivar Sjaastad; Finn Olav Levy; Tor Skomedal; Jan-Bjørn Osnes; Eirik Qvigstad
Journal:  Br J Pharmacol       Date:  2011-01       Impact factor: 8.739

4.  Differential regulation of C-type natriuretic peptide-induced cGMP and functional responses by PDE2 and PDE3 in failing myocardium.

Authors:  Lise Román Moltzau; Silja Meier; Jan Magnus Aronsen; Faraz Afzal; Ivar Sjaastad; Tor Skomedal; Jan-Bjørn Osnes; Finn Olav Levy; Eirik Qvigstad
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2014-01-15       Impact factor: 3.000

5.  5-HT4-elicited positive inotropic response is mediated by cAMP and regulated by PDE3 in failing rat and human cardiac ventricles.

Authors:  F Afzal; K W Andressen; H K Mørk; J M Aronsen; I Sjaastad; C P Dahl; T Skomedal; F O Levy; J-B Osnes; E Qvigstad
Journal:  Br J Pharmacol       Date:  2008-09-01       Impact factor: 8.739

6.  Non-classical regulation of β1- and β 2-adrenoceptor-mediated inotropic responses in rat heart ventricle by the G protein Gi.

Authors:  Caroline Bull Melsom; Rizwan Iqbal Hussain; Øivind Ørstavik; Jan Magnus Aronsen; Ivar Sjaastad; Tor Skomedal; Jan-Bjørn Osnes; Finn Olav Levy; Kurt Allen Krobert
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2014-09-13       Impact factor: 3.000

7.  Phorbol esters induce synthesis of thromboplastin activity in human monocytes.

Authors:  T Lyberg; H Prydz
Journal:  Biochem J       Date:  1981-03-15       Impact factor: 3.857

8.  Mechanisms involved in PGE2-induced transactivation of the epidermal growth factor receptor in MH1C1 hepatocarcinoma cells.

Authors:  Ingun Heiene Tveteraas; Kristin Meisdalen Müller; Monica Aasrum; John Ødegård; Olav Dajani; Tormod Guren; Dagny Sandnes; Thoralf Christoffersen
Journal:  J Exp Clin Cancer Res       Date:  2012-09-11

9.  Gi proteins regulate adenylyl cyclase activity independent of receptor activation.

Authors:  Caroline Bull Melsom; Øivind Ørstavik; Jan-Bjørn Osnes; Tor Skomedal; Finn Olav Levy; Kurt Allen Krobert
Journal:  PLoS One       Date:  2014-09-09       Impact factor: 3.240

10.  Inhibitory effects of prostaglandin E2 on collagen synthesis and cell proliferation in human stellate cells from pancreatic head adenocarcinoma.

Authors:  Ewa Pomianowska; Dagny Sandnes; Krzysztof Grzyb; Aasa R Schjølberg; Monica Aasrum; Ingun H Tveteraas; Vegard Tjomsland; Thoralf Christoffersen; Ivar P Gladhaug
Journal:  BMC Cancer       Date:  2014-06-09       Impact factor: 4.430
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