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Literature DB >> 6244593

Crystallization of part of the mitochondrial electron transfer chain: cytochrome c oxidase--cytochrome c complex.

Abstract

Cytochrome c oxidase (ferrocytochrome c oxygen oxidoreductase, EC 1.9.3.1) was purified from beef heart mitochondria by affinity chromatography. Phospholipids were removed by washing the oxidase with detergent on the affinity column; 1 mole of cardiolipin remained per mole of heme a. The oxidase was mixed with excess cytochrome c in 1.5% (wt/vol) cholate to form a complex. Slow removal of the detergent from the mixture by dialysis resulted in crystallization of cytochrome oxidase in the form of a 1:1 complex with cytochrome c. The chemical composition and spectrophotometric properties of the crystal are described. Increasing the solubility of a hydrophobic membrane protein by combination with hydropholic ligand is demonstrated as a maneuver for crystallizing the membrane protein.

Entities: Chemical Gene

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Year: 1980 PMID： 6244593 PMCID： PMC348395 DOI： 10.1073/pnas.77.2.928

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

13 in total

Review 7. Conformational coupling in H+-pumps and ATP synthesis--its analysis with anisotropic inhibitors of energy transduction in oxidative phosphorylation.

Authors: T Higuti
Journal: Mol Cell Biochem Date: 1984 Impact factor: 3.396

8. Crystallization of reaction center from Rhodopseudomonas sphaeroides: preliminary characterization.

Authors: J P Allen; G Feher
Journal: Proc Natl Acad Sci U S A Date: 1984-08 Impact factor: 11.205

9. Purification and crystallization of the calcium binding protein of sarcoplasmic reticulum from skeletal muscle.

Authors: A Maurer; M Tanaka; T Ozawa; S Fleischer
Journal: Proc Natl Acad Sci U S A Date: 1985-06 Impact factor: 11.205

9 in total

Crystallization of part of the mitochondrial electron transfer chain: cytochrome c oxidase--cytochrome c complex.

1. A rapid method for the preparation of highly purified cytochrome oxidase.

2. Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system.

3. [Thin layer chromatography of phosphatides and glycolipids].

4. Studies on cytochrome oxidase. III. Improved preparation and some properties.

5. Studies of the electron transport system. XXXVI. Properties of copper in cytochroma oxidase.

6. Spectrum of horse-heart cytochrome c.

7. [Colorimetric micro determination of ammoniacal nitrogen].

8. Purification of cytochrome oxidase by using Sepharose-bound cytochrome c.

9. Studies on cytochrome cl. II. Oxidation mechanism of cytochrome c1 in the presence of cytochromes a and c1.

10. Quantitative analysis of phospholipids by thin-layer chromatography.

1. Frequencies of hydrophobic and hydrophilic runs and alternations in proteins of known structure.

Review 2. Strategies in the reassembly of membrane proteins into lipid bilayer systems and their functional assay.

Review 3. Binding energy, conformational change, and the mechanism of transmembrane solute movements.

4. Crystallization of intrinsic membrane proteins.

5. Crystallization of the middle part of the mitochondrial electron transfer chain: cytochrome bc1-cytochrome c complex.

6. Crystallization of mitochondrial cytochrome oxidase.

Review 7. Conformational coupling in H+-pumps and ATP synthesis--its analysis with anisotropic inhibitors of energy transduction in oxidative phosphorylation.

8. Crystallization of reaction center from Rhodopseudomonas sphaeroides: preliminary characterization.

9. Purification and crystallization of the calcium binding protein of sarcoplasmic reticulum from skeletal muscle.