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Literature DB >> 6244580

Organization of kappa light chain genes in germ-line and somatic tissue.

R Joho, I L Weissman, P Early, J Cole, L Hood.

Abstract

We studied the organization of the kappa light chain genes in germ-line (sperm) and somatic (embryo) tissues. We constructed a plasmid containing a DNA insert coding for the kappa chain MOPC 167 and used the Southern blotting technique to determine the organization of kappa variable and constant region genes. In the haploid genome of the mouse there is only one constant region gene detectable and it has the same organization in sperm and embryo DNAs. There are several variable region genes in sperm and embryo that are related to the Vk167 gene. The organization of the V genes in sperm and embryo DNAs is identical. These results show that there is no rearrangement of variable region genes (or "minigenes") during early embryogenesis.

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Year: 1980 PMID： 6244580 PMCID： PMC348433 DOI： 10.1073/pnas.77.2.1106

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

40 in total

1. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.

Authors: P H Seeburg; J Shine; J A Martial; J D Baxter; H M Goodman
Journal: Nature Date: 1977-12-08 Impact factor: 49.962

2. Cloning of an immunoglobulin variable region gene from mouse embryo.

Authors: S Tonegawa; C Brack; N Hozumi; R Schuller
Journal: Proc Natl Acad Sci U S A Date: 1977-08 Impact factor: 11.205

3. Variability in the lambda light chain sequences of mouse antibody.

Authors: M G Weigert; I M Cesari; S J Yonkovich; M Cohn
Journal: Nature Date: 1970-12-12 Impact factor: 49.962

4. Quantitation of constant and variable region genes for mouse immunoglobulin lambda chains.

Authors: T Honjo; S Packman
Journal: Biochemistry Date: 1976-06-29 Impact factor: 3.162

5. No detectable reiteration of genes coding for mouse MOPC 41 immunoglobulin light-chain mRNA.

Authors: M G Farace; M F Aellen; P A Briand; C H Faust; P Vassalli; B Mach
Journal: Proc Natl Acad Sci U S A Date: 1976-03 Impact factor: 11.205

6. Demonstration that a mouse immunoglobulin light chain messenger RNA hybridizes exclusively with unique DNA.

Authors: T H Rabbitts; J M Jarvis; C Milstein
Journal: Cell Date: 1975-09 Impact factor: 41.582

7. Restriction endonuclease mapping of pSC101 and pMB9.

Authors: R C Tait; H W Boyer
Journal: Mol Gen Genet Date: 1978-09-08

8. The isolation of structural genes from libraries of eucaryotic DNA.

Authors: T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal: Cell Date: 1978-10 Impact factor: 41.582

9. Origin of antibody variation.

Authors: S Brenner; C Milstein
Journal: Nature Date: 1966-07-16 Impact factor: 49.962

10. Evidence supporting somatic assembly of the DNA segments (minigenes), coding for the framework, and complementarity-determining segments of immunoglobulin variable regions.

Authors: E A Kabat; T T Wu; H Bilofsky
Journal: J Exp Med Date: 1979-06-01 Impact factor: 14.307

29 in total

8. Chromosomal position and specific demethylation in enhancer sequences of germ line-transmitted retroviral genomes during mouse development.

Authors: D Jähner; R Jaenisch
Journal: Mol Cell Biol Date: 1985-09 Impact factor: 4.272

9. Variations in DNA methylation during mouse cell differentiation in vivo and in vitro.

Authors: A Razin; C Webb; M Szyf; J Yisraeli; A Rosenthal; T Naveh-Many; N Sciaky-Gallili; H Cedar
Journal: Proc Natl Acad Sci U S A Date: 1984-04 Impact factor: 11.205

10. A new means of inducibly inactivating a cellular protein.

Authors: J R Carlson
Journal: Mol Cell Biol Date: 1988-06 Impact factor: 4.272

Organization of kappa light chain genes in germ-line and somatic tissue.

1. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.

2. Cloning of an immunoglobulin variable region gene from mouse embryo.

3. Variability in the lambda light chain sequences of mouse antibody.

4. Quantitation of constant and variable region genes for mouse immunoglobulin lambda chains.

5. No detectable reiteration of genes coding for mouse MOPC 41 immunoglobulin light-chain mRNA.

6. Demonstration that a mouse immunoglobulin light chain messenger RNA hybridizes exclusively with unique DNA.

7. Restriction endonuclease mapping of pSC101 and pMB9.

8. The isolation of structural genes from libraries of eucaryotic DNA.

9. Origin of antibody variation.

10. Evidence supporting somatic assembly of the DNA segments (minigenes), coding for the framework, and complementarity-determining segments of immunoglobulin variable regions.

1. The endogenous Mtv-8 provirus resides within the V kappa locus.

2. Evolution of a V kappa gene family.

3. The mouse immunoglobulin kappa light-chain genes are located in early- and late-replicating regions of chromosome 6.

4. Misalignment of V and J gene segments resulting in a nonfunctional immunoglobulin gene.

5. An experimental approach to enumerate the genes coding for immunoglobulin variable-regions.

6. Two different IFN-gamma nonresponsive variants derived from the B-cell lymphoma 70Z/3.

7. Myeloma with multiple rearranged immunoglobulin kappa genes: only one kappa gene codes for kappa chains.

8. Chromosomal position and specific demethylation in enhancer sequences of germ line-transmitted retroviral genomes during mouse development.

9. Variations in DNA methylation during mouse cell differentiation in vivo and in vitro.

10. A new means of inducibly inactivating a cellular protein.