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Literature DB >> 6244549

Isolation of a replication origin complex from Escherichia coli.

K Nagai, W Hendrickson, R Balakrishnan, H Yamaki, D Boyd, M Schaechter.

Abstract

A complex consisting of replicative origin DNA and several proteins was isolated from Escherichia coli. Cells of temperature-sensitive mutants were labeled at the origin and fractionated by sucrose gradient centrifugation. A complex highly purified in origin DNA sedimented as a unique band. This complex dissociated at high concentration, above 0.2 M KCl. Upon dialysis, the complex reformed, allowing further purification of its constituents. Three major protein bands were found, corresponding to proteins of the outer membrane. The complex did not sediment with membrane fractions, but adhered to the outer membrane in the presence of magnesium.

Entities: Chemical Species

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Year: 1980 PMID： 6244549 PMCID： PMC348249 DOI： 10.1073/pnas.77.1.262

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

1. Organization of the replication-origin region of the Bacillus subtilis chromosome.

Authors: H Yoshikawa; K Yamaguchi; M Seiki; N Ogasawara; H Toyoda
Journal: Cold Spring Harb Symp Quant Biol Date: 1979

2. Deoxyribonucleic acid-envelope complexes isolated from Escherichia coli by free-flow electrophoresis: biochemical and electron microscope characterization.

Authors: W L Olsen; H G Heidrich; K Hannig; P H Hofschneider
Journal: J Bacteriol Date: 1974-05 Impact factor: 3.490

3. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

Authors: M J Osborn; J E Gander; E Parisi; J Carson
Journal: J Biol Chem Date: 1972-06-25 Impact factor: 5.157

4. Chromosomal sites of DNA-membrane attachment in Escherichia coli.

Authors: D L Parker; D A Glaser
Journal: J Mol Biol Date: 1974-08-05 Impact factor: 5.469

5. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

Isolation of a replication origin complex from Escherichia coli.

1. Organization of the replication-origin region of the Bacillus subtilis chromosome.

2. Deoxyribonucleic acid-envelope complexes isolated from Escherichia coli by free-flow electrophoresis: biochemical and electron microscope characterization.

3. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

4. Chromosomal sites of DNA-membrane attachment in Escherichia coli.

5. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

6. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

7. Escherichia coli mutants with temperature-sensitive synthesis of DNA.

8. Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli.

9. Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli.

10. Isolation of a cell membrane-DNA-nascent RNA complex from bacteria.

1. Characterization of the Escherichia coli membrane domain responsible for binding oriC DNA.

2. The 55-kilodalton protein in an oriC complex fraction is glycogen synthase.

3. Gene organization in the region containing a new gene involved in chromosome partition in Escherichia coli.

4. Asymmetric replication of an oriC plasmid in Escherichia coli.

5. Attachment of the chromosomal terminus of Bacillus subtilis to a fast-sedimenting particle.

6. Recognition sites for a membrane-derived DNA binding protein preparation in the E. coli replication origin.

7. Direct evidence for specific binding of the replicative origin of the Escherichia coli chromosome to the membrane.

8. Affinity of two different regions of the chromosome to the outer membrane of Escherichia coli.

9. Identification of specific restriction fragments associated with a membrane subparticle from Bacillus subtilis.

10. Membrane-DNA attachment sites in Streptococcus faecalis cells grown at different rates.