Literature DB >> 6244124

A direct, rapid, radioassay for adenosine diphosphatase.

G Smith, G D Smith, T J Peters.   

Abstract

Radiolabelled adenosine diphosphate [beta 32Pi]ADP was synthesized from radioactive orthophosphate and adenosine-5'-monophosphate with dicyclohexylcarbodiimide. The purified radioactive adenosine diphosphate was used as the substrate in a rapid, direct, one-step assay for adenosine diphosphatase in human neutrophilic leukocyte homogenates. Certain kinetic properties of the enzyme activity have been determined.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 6244124     DOI： 10.1016/0009-8981(80)90255-7

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  5 in total

1.  Studies on the kinetic properties and subcellular localization of adenosine diphosphatase activity in human peripheral blood lymphocytes.

Authors:  G P Smith; T Shah; A D Webster; T J Peters
Journal:  Clin Exp Immunol       Date:  1981-11       Impact factor: 4.330

2.  Ultrastructural localization of adenosine diphosphatase activity in cultured aortic endothelial cells.

Authors:  P D Wilson; G E Lieberman; T J Peters
Journal:  Histochem J       Date:  1982-03

3.  Studies on the kinetic properties and subcellular localization of adenine nucleotide phosphatases in peripheral blood lymphocytes from control subjects and patients with common variable primary hypogammaglobulinaemia.

Authors:  G P Smith; T Shah; A D Webster; T J Peters
Journal:  Clin Exp Immunol       Date:  1982-08       Impact factor: 4.330

4.  Electron microscopic cytochemical localization of nucleoside phosphatases in normal and chronic granulocytic leukaemic human neutrophils.

Authors:  P D Wilson; G J Rustin; G P Smith; T J Peters
Journal:  Histochem J       Date:  1981-01

5.  Subcellular localization and properties of rat liver adenosine diphosphatase.

Authors:  G P Smith; G D Smith; T J Peters
Journal:  Biochem J       Date:  1980-11-15       Impact factor: 3.857
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.