Literature DB >> 624373

Purification of the cytosol oestradiol-receptor complex from foetal guinea-pig uterus using electrofocusing on polyacrylamide plates.

J R Pasqualini, C Cosquer-Clavreul.   

Abstract

The 3H-oestradiol receptor complex obtained from the cytosol fraction of foetal guinea-pig uterus was purified by the following steps: column chromatography in Sephadex G-15 and Ultrogel, and by electrofocusing on polyacrylamide plates. In the final step a concentration of 15-17% of the foetal uterine oestradiol receptor protein was obtained. The isoelectric point (pl) of this receptor was determined to be 6.1-6.2.

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Year:  1978        PMID： 624373     DOI： 10.1007/bf01944721

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  5 in total

1.  Specific [3H]-estradiol binding in the fetal uterus and testis of guinea pig. Quantitative evolution of [3H]-estradiol receptors in the different fetal tissues (kidney, lung, uterus and testis) during fetal development.

Authors:  J R Pasqualini; C Sumida; C Gelly; B L Nguyen
Journal:  J Steroid Biochem       Date:  1976 Nov-Dec       Impact factor: 4.292

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Affinity chromatography and the purification of estrogen receptors.

Authors:  V Sica; I Parikh; E Nola; G A Puca; P Cuatrecasas
Journal:  J Biol Chem       Date:  1973-09-25       Impact factor: 5.157

4.  Estrogen-binding proteins of calf uterus. Interrelationship between various forms and identification of a receptor-transforming factor.

Authors:  G A Puca; E Nola; V Sica; F Bresciani
Journal:  Biochemistry       Date:  1972-10-24       Impact factor: 3.162

5.  [Cytosolic and nuclear estradiol receptors in the fetal guinea pig uterus].

Authors:  J R Pasqualini; B L Nguyen
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1976-09-13
  5 in total

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