B cell antigens of the HLA system: a simple serotyping technique based on non-cytotoxic anti-beta2-microglobulin reagents.

A modification of the NIH cytotoxicity test for recognizing B cell (D-associated) antigens and antibodies, when sera also contain anti-HLA(ABC) activity is described. The method is based on the observation that anti-beta2-microglobulin reagents are able to block lympholysis only when due to HLA(ABC) antigens.