Literature DB >> 62391

Studies on antibodies to histones by immunofluorescence.

E M Tan, J Robinson, P Robitaille.   

Abstract

When mouse kidney tissue sections were extracted with 0.1 N hydrochloric acid, sera with antibodies to certain nuclear antigens no longer stained tissue nuclei by immunofluorescence. This effect was due to removal of histones and nuclear acidic proteins Sm and nuclear ribonucleoprotein by the acid. DNA remained in the nuclei of the acid-extracted tissue sections. When solutions of calf thymus histones were reacted with acid-extracted tissues, histones combined with nuclear DNA to form complexes of DNA-histone. These complexes contained antigenic determinants which reacted with sera containing antibodies to deoxyribonucleoprotein to give nuclear staining demonstrated by immunofluorescence. The reaction was immunologically specific in that sera with antibodies to Sm and nuclear ribonucleoprotein were not reactive with reconstituted DNA-histone in nuclei. Other basic proteins such as protamine, poly-L-lysine, and poly-L-arginine could not substitute for histones. The method is introduced as a specific and reproducible assay for study of antibodies to histones.

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Year:  1976        PMID: 62391     DOI: 10.1111/j.1365-3083.1976.tb03030.x

Source DB:  PubMed          Journal:  Scand J Immunol        ISSN: 0300-9475            Impact factor:   3.487


  16 in total

Review 1.  Juvenile chronic arthritis, chronic iridocyclitis, and reactivity to histones.

Authors:  A M Leak; P Woo
Journal:  Ann Rheum Dis       Date:  1991-09       Impact factor: 19.103

Review 2.  Clinical relevance of autoantibodies in systemic rheumatic diseases.

Authors:  M J Fritzler
Journal:  Mol Biol Rep       Date:  1996       Impact factor: 2.316

3.  Anti-H1 histone antibodies in systemic lupus erythematosus: epitope localization after immunoblotting of chymotrypsin-digested H1.

Authors:  O Costa; J C Tchouatcha-Tchouassom; B Roux; J C Monier
Journal:  Clin Exp Immunol       Date:  1986-03       Impact factor: 4.330

4.  Cross-reacting rheumatoid factors and lupus erythematosus (LE)-factors.

Authors:  K Hannestad; O P Rekvig; A Husebekk
Journal:  Springer Semin Immunopathol       Date:  1981

5.  Characterisation of non-histone nuclear proteins cross reactive with purified rheumatoid factors.

Authors:  J C Mason; P J Venables; P R Smith; R N Maini
Journal:  Ann Rheum Dis       Date:  1985-05       Impact factor: 19.103

6.  Glomerular lesions induced in the rabbit by physicochemically altered homologous IgG.

Authors:  F Cavalot; M Miyata; A Vladutiu; V Terranova; S Dubiski; R Burlingame; E Tan; J Brentjens; F Milgrom; G Andres
Journal:  Am J Pathol       Date:  1992-03       Impact factor: 4.307

7.  A monoclonal antibody to the surface membrane of human platelets which inhibits ristocetin- and collagen-induced platelet aggregation reacts with H1 histones of cell nuclei.

Authors:  L Cosgrove; F Alderuccio; B H Toh; J Pedersen; A Holliday; I McKenzie
Journal:  Clin Exp Immunol       Date:  1987-08       Impact factor: 4.330

8.  Antinuclear antibodies and the diagnosis of systemic lupus erythematosus in patients with acute intermittent porphyria.

Authors:  S A Allard; P J Charles; A L Herrick; K E McColl; J T Scott
Journal:  Ann Rheum Dis       Date:  1990-04       Impact factor: 19.103

9.  Human-human hybridoma autoantibodies with both anti-DNA and rheumatoid factor activities.

Authors:  J Rauch; H Tannenbaum; K Straaton; H Massicotte; J Wild
Journal:  J Clin Invest       Date:  1986-01       Impact factor: 14.808

10.  Immunoelectronmicroscopy of metaphase chromosomes.

Authors:  F T Bosman; P K Nakane
Journal:  Histochemistry       Date:  1982
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